Characterization of nanoparticle uptake by endothelial cells
Characterization of nanoparticle uptake by endothelial cells
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DOI：
10.1016/s0378-5173(01)00923-1
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发表时间：
2002-02-21
影响因子：
5.8
通讯作者：
Labhasetwar, V
中科院分区：
文献类型：
--
作者：
Davda, J;Labhasetwar, V
由于内皮素在生物系统中的重要作用，它是药物或基因治疗的重要靶点。在本文中，我们的特点是纳米颗粒摄取内皮细胞在细胞培养。使用含有牛血清白蛋白的聚DL-丙交酯-共-乙交酯聚合物作为模型蛋白和6-香豆素作为荧光标记物来配制纳米颗粒。观察到纳米颗粒的细胞摄取取决于孵育时间和培养基中纳米颗粒的浓度。共聚焦显微镜下纳米颗粒的摄取是快速的，表明它们主要定位在细胞质中。促有丝分裂研究证明了纳米颗粒与细胞的生物相容性。因此，该研究表明，纳米颗粒可用于将治疗剂或基因定位到内皮细胞中。纳米粒定位于内皮细胞中，由于其持续释放特性，可以提供延长的药物作用，并且还可以保护包封的药物免受酶降解。(C)2002 Elsevier Science B. V.保留所有权利。
Endothelium is an important target for drug or gene therapy because of its important role in the biological system. In this paper, we have characterized nanoparticle uptake by endothelial cells in cell culture. Nanoparticles were formulated Using poly DL-lactide-co-glycolide polymer containing bovine serum albumin as a model protein and 6-coumarin as a fluorescent marker. It was observed that the cellular uptake of nanoparticles depends on the time of incubation and the concentration of nanoparticles in the medium. The uptake of nanoparticles was rapid with confocal microscopy demonstrating their localization mostly in the cytoplasm. The mitogenic study demonstrated biocompatability of nanoparticles with the cells. The study thus demonstrates that nanoparticles could be used for localizing therapeutic agents or gene into endothelial cells. Nanoparticles localized in the endothelium could provide prolonged drug effects because of their sustained release characterics, and also could protect the encapsulated agent from enzymatic degradation. (C) 2002 Elsevier Science B.V. All rights reserved.