Drosophila seminal protein ovulin mediates ovulation through female octopamine neuronal signaling
Drosophila seminal protein ovulin mediates ovulation through female octopamine neuronal signaling
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DOI：
10.1073/pnas.1220018110
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发表时间：
2013-10-22
影响因子：
11.1
通讯作者：
Wolfner, Mariana F.
中科院分区：
文献类型：
--
作者：
Rubinstein, C. Dustin;Wolfner, Mariana F.
在整个动物类群中，精液蛋白是雌性生殖生理和行为的重要调节因素。然而，精液蛋白影响这些变化的生理或分子机制却知之甚少。为了研究这个问题，我们研究了交配诱导的果蝇排卵行为的增加。排卵需要来自中枢神经系统的章鱼胺(OA)信号来协调卵子从卵巢释放并进入输卵管。精液中的卵蛋白可提高交配后的排卵率。我们测试了卵蛋白是否通过卵泡刺激素来增加排卵行为。增加的OA神经元兴奋性弥补了在交配过程中接收到的卵蛋白的缺乏。此外，我们发现了一种交配依赖性的输卵管肌肉松弛，对于这种松弛来说，卵子是男性的必要和充分的贡献。我们进一步报道，输卵管肌肉松弛可以通过激活OA神经元来诱导，这需要OA正常的代谢产物，并反映了卵蛋白对OA神经元信号的增加。最后，我们证明了卵蛋白暴露的结果是，在输卵管中有随后的OA突触部位的生长，这表明精液蛋白可以促进突触的可塑性。综上所述，这些结果表明，卵蛋白通过OA神经元信号促进排卵，进而表明，精液蛋白可以通过调节已知的女性生殖途径来改变生殖生理。
Across animal taxa, seminal proteins are important regulators of female reproductive physiology and behavior. However, little is understood about the physiological or molecular mechanisms by which seminal proteins effect these changes. To investigate this topic, we studied the increase in Drosophila melanogaster ovulation behavior induced by mating. Ovulation requires octopamine (OA) signaling from the central nervous system to coordinate an egg's release from the ovary and its passage into the oviduct. The seminal protein ovulin increases ovulation rates after mating. We tested whether ovulin acts through OA to increase ovulation behavior. Increasing OA neuronal excitability compensated for a lack of ovulin received during mating. Moreover, we identified a mating-dependent relaxation of oviduct musculature, for which ovulin is a necessary and sufficient male contribution. We report further that oviduct muscle relaxation can be induced by activating OA neurons, requires normal metabolic production of OA, and reflects ovulin's increasing of OA neuronal signaling. Finally, we showed that as a result of ovulin exposure, there is subsequent growth of OA synaptic sites at the oviduct, demonstrating that seminal proteins can contribute to synaptic plasticity. Together, these results demonstrate that ovulin increases ovulation through OA neuronal signaling and, by extension, that seminal proteins can alter reproductive physiology by modulating known female pathways regulating reproduction.