The C. elegans septin genes, unc-59 and unc-61, are required for normal postembryonic cytokineses and morphogenesis but have no essential function in embryogenesis.
The C. elegans septin genes, unc-59 and unc-61, are required for normal postembryonic cytokineses and morphogenesis but have no essential function in embryogenesis.
复制标题
DOI：
--
复制
发表时间：
2000-11
影响因子：
4
通讯作者：
Tri Q. Nguyen;Hitoshi Sawa;Hideyuki Okano;John G. White
中科院分区：
文献类型：
--
作者：
Tri Q. Nguyen;Hitoshi Sawa;Hideyuki Okano;John G. White
已经证明，隔膜蛋白在从酵母到哺乳动物的多种生物体中的胞质分裂中发挥重要作用。在这项研究中，我们发现，unc-59和unc-61位点编码秀丽隐杆线虫septins。基因组数据库检索表明，unc-59和unc-61可能是C.线虫基因组<$-59和<$-61定位于卵裂沟的前缘，并最终驻留在中体。对unc-59和unc-61突变体的分析表明，每一个septin都需要另一个septin的存在才能定位到细胞动力学沟。令人惊讶的是，unc-59和unc-61突变体通常具有正常的胚胎发育;然而，在胚胎后发育中观察到缺陷，影响外阴，雄性尾巴，性腺和感觉神经元的形态发生。这些缺陷可以至少部分归因于胚胎后细胞分裂素的失败，虽然我们的数据也表明septins的其他可能的作用。UNC-59和UNC-61双突变体显示出与每个单突变体相似的缺陷。
Septins have been shown to play important roles in cytokinesis in diverse organisms ranging from yeast to mammals. In this study, we show that both the unc-59 and unc-61 loci encode Caenorhabditis elegans septins. Genomic database searches indicate that unc-59 and unc-61 are probably the only septin genes in the C. elegans genome. UNC-59 and UNC-61 localize to the leading edge of cleavage furrows and eventually reside at the midbody. Analysis of unc-59 and unc-61 mutants revealed that each septin requires the presence of the other for localization to the cytokinetic furrow. Surprisingly, unc-59 and unc-61 mutants generally have normal embryonic development; however, defects were observed in post-embryonic development affecting the morphogenesis of the vulva, male tail, gonad, and sensory neurons. These defects can be at least partially attributed to failures in post-embryonic cytokineses although our data also suggest other possible roles for septins. unc-59 and unc-61 double mutants show similar defects to each of the single mutants.