Murine xenogeneic models of myelodysplastic syndrome: an essential role for stroma cells.
Murine xenogeneic models of myelodysplastic syndrome: an essential role for stroma cells.
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DOI：
10.1016/j.exphem.2013.10.002
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发表时间：
2014-01
影响因子：
2.6
通讯作者：
Deeg HJ
中科院分区：
文献类型：
--
作者：
Li X;Deeg HJ
综述骨髓增生异常综合征（MDS）的小鼠异种移植模型。文献综述，实验策略在免疫缺陷小鼠中实现MDS细胞持续植入的困难可能在于MDS克隆的先天特征和微环境因素。通过静脉内（IV）注射实现了极低数量的CD 45+克隆MDS细胞的植入;通过髓内途径获得了更高的植入率。某些基质组分与造血细胞的共注射克服了IV给药的局限性，允许高比例的人CD 45+细胞在小鼠脾脏和骨髓中植入。基质细胞上的CD 146表达传达了移植促进作用。克隆MDS细胞已繁殖超过6个月的时间，并已成功移植到第二受体。通过共注射基质/间充质细胞，特别是IV给药，大大促进了具有干细胞特征的人克隆MDS细胞在免疫缺陷小鼠中的移植;基质上的CD 146表达是一个重要因素。然而，没有模型开发人MDS的实验室和临床特征。需要进一步的工作来确定MDS全面演变所需的细胞和非细胞因素。
Review murine xenotransplantation models for myelodysplastic syndromes (MDS). Literature review, experimental strategies The difficulties in achieving sustained engraftment of MDS cells in immunodeficient mice may lie in innate characteristics of the MDS clone(s) and microenvironmental factors. Engraftment of very low numbers of CD45+ clonal MDS cells has been achieved with intravenous (IV) injection; higher rates of engraftment are obtained via the intramedullary route. Co-injection of certain stroma components with hematopoietic cells overcomes limitations of IV administration, allowing for engraftment of high proportions of human CD45+ cells in mouse spleen and marrow. Expression of CD146 on stroma cells conveys an engraftment-facilitating effect. Clonal MDS cells have been propagated for periods beyond 6 months and have been transplanted successfully into secondary recipients. Engraftment of human clonal MDS cells with stem cell characteristics in immunodeficient mice is greatly facilitated by co-injection of stroma/mesenchymal cells, particularly with IV administration; CD146 expression on stroma is an essential factor. However, no model develops the laboratory and clinical features of human MDS. Additional work is needed to determine cellular and non-cellular factors required for the full evolution of MDS.
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影响因子：
20.3
作者：
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影响因子：
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作者：
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影响因子：
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作者：
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