Assessment of Thermal Pain Sensation in Rats and Mice Using the Hargreaves Test
Assessment of Thermal Pain Sensation in Rats and Mice Using the Hargreaves Test
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DOI：
10.21769/bioprotoc.2506
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发表时间：
2017-08-20
期刊：
影响因子：
0.8
通讯作者：
Andrews, Melissa R.
中科院分区：
文献类型：
--
作者：
Cheah, Menghon;Fawcett, James W.;Andrews, Melissa R.
Hargreaves测试专门设计用于评估啮齿动物（如大鼠和小鼠）的热痛觉。该测试已用于涉及神经损伤和再生后的疼痛致敏或热疼痛反应恢复的实验中。我们在这里提出了一个循序渐进的协议，并强调了重要的注意事项，以指导首次使用者完成学习过程。此外，我们还包括代表性的数据，从大鼠模型的感觉去神经，显示如何数据可以进行分析，以获得有意义的结果。我们希望该方案也可以帮助潜在用户决定Hargreaves测试是否适合他们的实验。
The Hargreaves test is specifically designed to assess thermal pain sensation in rodents such as rats and mice. This test has been used in experiments involving pain sensitization or recovery of thermal pain response following neural injury and regeneration. We present here a step-by-step protocol highlighted with important notes to guide first-time users through the learning process. Additionally, we have also included representative data from a rat model of sensory denervation showing how the data can be analysed to obtain meaningful results. We hope that this protocol can also assist potential users in deciding whether the Hargreaves test is a suitable test for their experiment.