Combination of BCL11A siRNA with vincristine increases the apoptosis of SUDHL6 cells.
Combination of BCL11A siRNA with vincristine increases the apoptosis of SUDHL6 cells.
复制标题
BCL11A siRNA 与长春新碱的组合可增加 SUDHL6 细胞的凋亡
DOI：
10.1186/2047-783x-19-34
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发表时间：
2014-06-24
影响因子：
4.2
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
He D;Wu H;Ding L;Li Y
背景：B细胞慢性淋巴细胞白血病/淋巴瘤11 A（BCL 11 A）与人类B细胞恶性肿瘤的发生有关。我们以前的研究表明，通过小干扰RNA（siRNA）下调BCL 11 A mRNA能够诱导SUDHL 6细胞系的凋亡。为了进一步探讨BCL 11 A siRNA对化疗药物细胞毒作用的影响，我们研究了BCL 11 A siRNA联合长春新碱（VCR）对SUDHL 6细胞增殖和凋亡的影响。通过CCK 8测定法测量细胞增殖。Hoechst 33258染色观察凋亡细胞形态。结果：BCL 11 A siRNA联合VCR处理后，细胞增殖能力明显低于VCR、BCL 11 A siRNA单独处理组和阴性对照siRNA联合VCR处理组（P <0.05）;结论：BCL 11 A siRNA联合VCR能促进SUDHL 6细胞凋亡，与BCL 11 A siRNA和VCR单独处理及阴性对照siRNA联合VCR处理相比，差异有统计学意义（P <0.05）。我们的研究表明，BCL 11 A siRNA联合VCR可能是提高B细胞淋巴瘤有效治疗的一种有用的方法。
Background:B cell chronic lymphocytic leukemia/lymphoma 11 A (BCL11A) is associated with human B cell malignancy initiation. Our previous study has shown that downregulation of BCL11A mRNA by small interfering RNA (siRNA) is capable of inducing apoptosis in the SUDHL6 cell line. To further explore the effects of BCL11A siRNA on the enhanced cytotoxicity of a chemotherapeutic drug, we investigated the effects of BCL11A siRNA combined with vincristine (VCR) on SUDHL6 cell proliferation and apoptosis.Methods:Chemically synthesized BCL11A siRNA was transfected into SUDHL6 cells using the HiPerFect Transfection Reagent in combination with VCR. Cell proliferation was measured by the CCK8 assay. The morphology of apoptotic cells was observed with Hoechst 33258 staining. The rate of cell apoptosis was determined by annexin V-fluorescein isothiocyanate/propidium iodide double staining using fluorescence-activated cell sorting (FACS) analysis.Results:After BCL11A siRNA plus VCR treatment, cell proliferation was significantly decreased in comparison with VCR or BCL11A siRNA treatment alone and negative control siRNA plus VCR treatment (P <0.05). The apoptotic rate of BCL11A siRNA plus VCR treated cells was significantly increased compared with BCL11A siRNA and VCR treatment alone and negative control siRNA plus VCR treatment (P <0.05).Conclusions:The combination of BCL11A siRNA and VCR increases apoptosis in SUDHL6 cells. Our study implies that BCL11A siRNA in combination with VCR may be a useful approach for improving effective treatment for B cell lymphoma.