Malaria parasites giving rise to recrudescence in vitro
Malaria parasites giving rise to recrudescence in vitro
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DOI：
10.1128/aac.46.4.958-965.2002
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发表时间：
2002-04-01
影响因子：
4.9
通讯作者：
Kanbara, H
中科院分区：
文献类型：
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作者：
Nakazawa, S;Maoka, T;Kanbara, H
通过药物治疗在体外模拟复发，以检查药物敏感的寄生虫如何在治疗中存活。用致死剂量的乙胺嘧啶或甲氟喹处理不同数量的培养寄生虫不同时间长度。当治疗持续时间延长时，在具有较大初始寄生虫数量的寄生虫群体中观察到复发。用不同浓度的乙胺嘧啶或甲氟喹处理相同数量的寄生红细胞。复发发生率与药物浓度之间不存在明显的线性关系。已经复发的寄生虫在随后的复发实验中连续复发。从治疗停止到复发性寄生虫血症水平达到1%的时间以及复发性寄生虫的生长率在这些复发性寄生虫之间是相等的。这些实验中复发的寄生虫与治疗前测试的寄生虫一样对药物敏感。这些结果表明，寄生虫培养物可能含有处于某些阶段的寄生虫，这些寄生虫在长达 10 天的时间内都不会被药物杀死，这解释了即使在治疗后也会出现复发的原因。
Recrudescences were simulated in vitro with drug treatment to examine how drug-sensitive parasites survive the treatment. Various numbers of cultured parasites were treated with lethal doses of pyrimethamine or mefloquine for various lengths of time. Recrudescences were observed in parasite populations with larger initial numbers of parasites when the treatment duration was prolonged. Equal numbers of parasitized erythrocytes were treated with various concentrations of pyrimethamine or mefloquine. There was no clear linear relationship between the incidence of recrudescence and the drug concentration. Parasites that had recrudesced were continuously allowed to recrudesce in the succeeding recrudescence experiments. Both the duration from the cessation of treatment to the time at which the recrudescent parasitemia level reached 1% and the growth rate of recrudescent parasites were equal among these recrudescences. The recrudescent parasites in these experiments were as sensitive to the drugs as the parasites tested before treatment were. These results suggest that a parasite culture may contain parasites in some phases that are not killed by drug for up to 10 days, which explains the recrudescences that occur even after treatment.