X-ray-induced delay in the Chinese hamster cell-cycle: dependence on phase irradiated under different culturing conditions, BUdR incorporation, and hypertonic treatment.
X-ray-induced delay in the Chinese hamster cell-cycle: dependence on phase irradiated under different culturing conditions, BUdR incorporation, and hypertonic treatment.
复制标题
X 射线诱导的中国仓鼠细胞周期延迟：依赖于不同培养条件下照射的相位、BUdR 掺入和高渗处理。
DOI：
10.1080/09553007314550691
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
H. H. Miller
中科院分区：
文献类型：
--
作者：
D. Miller;W. Dewey;H. H. Miller
SummaryChinese hamster ovary (CHO) cells, synchronized by selecting mitotic cells, received 300 rads of X-radiation at various intervals during the cell-cycle and were examined for cell number until they had completed their next wave of division. These growth curves were analysed for division delay in relation to type of media, i.e. modified Ham's F-10 or modified McCoy's 5 a, and to culturing conditions, i.e. monolayer or suspension. Regardless of the type of media or culturing conditions used, mitotic cells were delayed somewhat longer (0·55 min/rad) than early-mid G1 cells, and then the delay increased with cell age from early-mid G1 (0·32 min/rad) to late S-early G2 (0·84 min/rad). Thus, radiosensitivity during the cell-cycle approaches in late S phase a maximum for division delay in contrast to a minimum for cell killing and chromosomal aberrations. Furthermore, division delay was not increased by either incorporating bromodeoxyuridine into the DNA or treating the cells with hypertonic medium, althou...
