Schwann cells are axo-protective after injury irrespective of myelination status in mouse Schwann cell-neuron cocultures.
Schwann cells are axo-protective after injury irrespective of myelination status in mouse Schwann cell-neuron cocultures.
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DOI：
10.1242/jcs.261557
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发表时间：
2023-09-15
影响因子：
4
通讯作者：
中科院分区：
文献类型：
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施万细胞(SC) -背根神经节（DRG）神经元共培养是了解周围神经系统（PNS）髓鞘形成、损伤和再生过程中细胞-细胞信号传导和相互作用的重要技术。虽然使用大鼠SCs和神经元或小鼠DRG外植体的方法是常见的，但目前还没有建立与游离小鼠细胞共培养的区隔化髓鞘的方案。因此，需要一种共培养方案，允许对小鼠SCs或神经元进行单独的遗传操作，或使用来自不同转基因动物的细胞来补充小鼠体内实验。然而，在培养中诱导解离的小鼠SCs形成髓鞘是具有挑战性的。在这里，我们描述了一种在微流体室中共培养游离小鼠SCs和DRG神经元并诱导强大髓鞘形成的新方法。共培养物可以被切开研究损伤并用于药物治疗，细胞可以被慢病毒转导用于实时成像。我们使用该模型研究创伤性轴突切开术后的轴突变性，发现无论髓鞘形成状态如何，SCs都具有轴突保护作用。在损伤后的较晚时间点，共培养的实时成像显示SCs破裂，摄取并清除轴突碎片。一种区隔化小鼠髓鞘化雪旺细胞- drg神经元共培养的新方法，表明雪旺细胞髓鞘化状态不影响其损伤后的轴突保护作用。
Myelinating Schwann cell (SC)–dorsal root ganglion (DRG) neuron cocultures are an important technique for understanding cell–cell signalling and interactions during peripheral nervous system (PNS) myelination, injury, and regeneration. Although methods using rat SCs and neurons or mouse DRG explants are commonplace, there are no established protocols for compartmentalised myelinating cocultures with dissociated mouse cells. There consequently is a need for a coculture protocol that allows separate genetic manipulation of mouse SCs or neurons, or use of cells from different transgenic animals to complement in vivo mouse experiments. However, inducing myelination of dissociated mouse SCs in culture is challenging. Here, we describe a new method to coculture dissociated mouse SCs and DRG neurons in microfluidic chambers and induce robust myelination. Cocultures can be axotomised to study injury and used for drug treatments, and cells can be lentivirally transduced for live imaging. We used this model to investigate axon degeneration after traumatic axotomy and find that SCs, irrespective of myelination status, are axo-protective. At later timepoints after injury, live imaging of cocultures shows that SCs break up, ingest and clear axonal debris. A novel method for compartmentalised mouse myelinating Schwann cell–DRG neuron cocultures, showing that Schwann cell myelination status does not influence their axon protective effect after injury.
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影响因子：
5.9
作者：
Boutary S;Caillaud M;El Madani M;Vallat JM;Loisel-Duwattez J;Rouyer A;Richard L;Gracia C;Urbinati G;Desmaële D;Echaniz-Laguna A;Adams D;Couvreur P;Schumacher M;Massaad C;Massaad-Massade L
通讯作者：
Massaad-Massade L
DOI：
10.1083/jcb.201205025
发表时间：
2012-07-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fontana X;Hristova M;Da Costa C;Patodia S;Thei L;Makwana M;Spencer-Dene B;Latouche M;Mirsky R;Jessen KR;Klein R;Raivich G;Behrens A
通讯作者：
Behrens A
轴突内部和胶质相关的轴突变性共同调节的分子分析。
影响因子：
9
作者：
Catenaccio A;Llavero Hurtado M;Diaz P;Lamont DJ;Wishart TM;Court FA
通讯作者：
Court FA
影响因子：
7.8
作者：
Einheber, S;Zanazzi, G;Ching, W;Scherer, S;Milner, TA;Peles, E;Salzer, JL
通讯作者：
Salzer, JL
影响因子：
16.6
作者：
Fernando RN;Cotter L;Perrin-Tricaud C;Berthelot J;Bartolami S;Pereira JA;Gonzalez S;Suter U;Tricaud N
通讯作者：
Tricaud N