Antibiotics at subinhibitory concentrations improve the quorum sensing behavior of Chromobacterium violaceum
Antibiotics at subinhibitory concentrations improve the quorum sensing behavior of Chromobacterium violaceum
复制标题
亚抑制浓度的抗生素可改善紫色色杆菌的群体感应行为。
DOI：
10.1111/1574-6968.12086
复制
发表时间：
2013-04-01
影响因子：
2.1
通讯作者：
Lu, Xinzhi
中科院分区：
文献类型：
--
作者：
Liu, Zhanjun;Wang, Weishan;Lu, Xinzhi
越来越多的证据表明，抗生素的功能是微生物间的信号分子，而不是杀伤性武器。然而，对这些功能所涉及的机制和关键因素仍然知之甚少。早期的发现将抗生素信号与群体感应（QS）联系起来;然而，结果在实验，抗生素和细菌菌株之间存在差异。在这项研究中，我们发现，在亚抑制浓度的抗生素提高了紫色色杆菌ATCC 12472的紫色素生产能力。QS相关基因转录物的定量实时聚合酶链反应和QS突变株中紫色素生产的生物测定表明，抗生素增强了N-酰基-L-高丝氨酸内酯（AHL; QS信号分子）的产生，并增加了AHL诱导的QS介导的毒力，包括几丁质酶的产生和生物膜的形成。此外，还发现了阳性鞭毛活性和增加的细菌聚集能力，这与抗生素诱导的生物膜形成有关。我们的研究结果表明，拟南芥介导的种间信号也发生在C。violaceum，从而扩展了细胞间交流的知识和语言。
Increasing evidence has shown that antibiotics function as intermicrobial signaling molecules instead of killing weapons. However, mechanisms and key factors that are involved in such functions remain poorly understood. Earlier findings have associated antibiotic signaling with quorum sensing (QS); however, results varied among experiments, antibiotics, and bacterial strains. In this study, we found that antibiotics at subinhibitory concentrations improved the violacein-producing ability of Chromobacterium violaceum ATCC 12472. Quantitative real-time polymerase chain reaction of QS-associated gene transcripts and bioassay of violacein production in a QS mutant strain demonstrated that antibiotics enhanced the production of N-acyl-L-homoserine lactones (AHLs; QS signaling molecules) and increased AHL-inducing QS-mediated virulence, including chitinase production and biofilm formation. Moreover, a positive flagellar activity and an increased bacterial clustering ability were found, which are related to the antibiotic-induced biofilm formation. Our findings suggested that antibiotic-mediated interspecific signaling also occurs in C. violaceum, thereby expanding the knowledge and language of cell-to-cell communication.