Metabolic interactions between animal cells through permeable intercellular junctions.
Metabolic interactions between animal cells through permeable intercellular junctions.
复制标题
动物细胞之间通过可渗透的细胞间连接进行代谢相互作用。
DOI：
10.1016/0014-4827(79)90427-0
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发表时间：
1979
影响因子：
3.7
通讯作者：
John D. Pitts
中科院分区：
文献类型：
--
作者：
Judson D. Sheridan;Malcolm E. Finbow;John D. Pitts
在HGPRT-（缺乏次黄嘌呤：鸟嘌呤磷酸核糖转移酶活性）和野生型哺乳动物细胞的混合培养物中，嘌呤核苷酸池在掺入核酸之前在两种细胞类型之间平衡。因此，在外源性次黄嘌呤的存在下，突变体细胞刺激野生型细胞中的HGPRT途径的活性，并且嘌呤核苷酸生物合成的从头途径在两种细胞类型中均被抑制。也就是说，偶联细胞的混合培养物的代谢活性是混合物的独特特征，而不仅仅是组分细胞的活性的总和，而不通过可渗透的细胞间连接偶联的细胞的混合培养物的活性仅仅是两种细胞类型的活性的总和。
In mixed cultures of HGPRT−(lacking hypoxanthine: guanine phosphoribosyltransferase activity) and wild-type mammalian cells coupled by permeable intercellular junctions, the purine nucleotide pools equilibrate between the two cell types prior to incorporation into nucleic acid. As a consequence, in the presence of exogenous hypoxanthine, the mutant cells stimulate the activity of HGPRT pathway in the wild-type cells and the de novo pathway of purine nucleotide biosynthesis is inhibited in both cell types. That is, the metabolic activity of a mixed culture of coupled cells is a unique characteristic of the mixture, not simply the sum of the activities of the component cells, whereas the activity of a mixed culture of cells which are not coupled by permeable intercellular junctions is simply the sum of the activities of the two cell types.