Epidermal Patch with Glucose Biosensor: pH and Temperature Correction toward More Accurate Sweat Analysis during Sport Practice
Epidermal Patch with Glucose Biosensor: pH and Temperature Correction toward More Accurate Sweat Analysis during Sport Practice
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DOI：
10.1021/acs.analchem.0c02211
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发表时间：
2020-07-21
影响因子：
7.4
通讯作者：
Crespo, Gaston A.
中科院分区：
文献类型：
--
作者：
Wiorek, Alexander;Parrilla, Marc;Crespo, Gaston A.
我们推出了一种用于汗液葡萄糖分析的表皮贴片，首次根据体内测试过程中汗液的局部动态波动进行 pH 和温度校正。这种校正确实是本文的主要新颖之处，对于每个基于酶元素的传感器的可靠测量至关重要，酶元素的活性很大程度上取决于 pH 值和温度。本文报告的在体测量期间校正葡萄糖检测的结果得到两步验证方案的支持：生物传感器在离体和体上操作，将结果与紫外可见光谱法和/或离子色谱法相关联。重要的是，该可穿戴设备是一个灵活的皮肤贴片，包括一个微流体单元，该单元设计有一个汗水收集区，耦合到一个流体通道，其中放置了所需的电极：葡萄糖生物传感器、pH 电位电极和温度传感器。葡萄糖生物传感器在汗液中葡萄糖的预期生理水平（10-200μM）内呈现线性响应范围，并且通过新的“校正方法”动态调整校准参数以适应运动练习期间pH和温度的任何变化。此外，该传感器还具有快速响应时间、适当的选择性和出色的可逆性。总共进行了 9 项经过验证的身体测试：结果揭示了可穿戴葡萄糖传感器在提供与体育活动期间的个人相关的可靠生理数据方面的巨大潜力。特别是，所开发的“校正方法”预计将影响下一代可穿戴设备，这些设备通过化学信息以可靠的方式将生理活动数字化，用于运动和医疗保健应用。
We present an epidermal patch for glucose analysis in sweat incorporating for the first time pH and temperature correction according to local dynamic fluctuations in sweat during on-body tests. This sort of correction is indeed the main novelty of the paper, being crucial toward reliable measurements in every sensor based on an enzymatic element whose activity strongly depends on pH and temperature. The results herein reported for corrected glucose detection during on-body measurements are supported by a two-step validation protocol: with the biosensor operating off- and on-bodily, correlating the results with UV-vis spectrometry and/or ion chromatography. Importantly, the wearable device is a flexible skin patch that comprises a microfluidic cell designed with a sweat collection zone coupled to a fluidic channel in where the needed electrodes are placed: glucose biosensor, pH potentiometric electrode and a temperature sensor. The glucose biosensor presents a linear range of response within the expected physiological levels of glucose in sweat (10-200 mu M), and the calibration parameters are dynamically adjusted to any change in pH and temperature during the sport practice by means of a new "correction approach". In addition, the sensor displays a fast response time, appropriate selectivity, and excellent reversibility. A total of 9 validated on-body tests are presented: the outcomes revealed a great potential of the wearable glucose sensor toward the provision of reliable physiological data linked to individuals during sport activity. In particular, the developed "correction approach" is expected to impact into the next generation of wearable devices that digitalize physiological activities through chemical information in a trustable manner for both sport and healthcare applications.