Purification, properties and regulation of the level of bovine S-adenosylmethionine decarboxylase during lymphocyte mitogenesis.
Purification, properties and regulation of the level of bovine S-adenosylmethionine decarboxylase during lymphocyte mitogenesis.
复制标题
淋巴细胞有丝分裂过程中牛 S-腺苷甲硫氨酸脱羧酶的纯化、性质和水平调节。
DOI：
10.1016/0304-4165(82)90265-3
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Morris,DR
中科院分区：
文献类型：
--
作者：
Seyfried,CE;Oleinik,OE;Degen,JL;Resing,K;Morris,DR
从用丙酮醛双（鸟苷酰腙）处理的小牛肝脏中纯化S-腺苷甲硫氨酸脱羧酶，以提高酶的水平。纯化的牛S-腺苷甲硫氨酸脱羧酶的比活性和亚基分子量（32 000）类似于以前从大鼠和小鼠分离的酶。牛肝酶免疫交叉反应与S-腺苷甲硫氨酸脱羧酶从休息和促有丝分裂激活的牛淋巴细胞。通过用[3 H]亮氨酸标记细胞并通过亲和层析和十二烷基硫酸钠凝胶电泳分离放射性脱羧酶来测定活化淋巴细胞中的酶合成速率。有丝分裂原处理后9小时，酶合成速率增加了10倍，这是细胞酶最初增加的原因。没有进一步增加的S-腺苷甲硫氨酸脱羧酶的合成率，与第二次升高的活动发生在约。促有丝分裂活化后24 h。得出结论，酶活性的第二次增加是由于酶的细胞内半衰期延长了2倍。
S-Adenosylmethionine decarboxylase was purified from the livers of calves treated with methylglyoxal bis (guanylhydrazone) to elevate the level of the enzyme. Purified bovineS-adenosylmethionine decarboxylase was similar in specific activity and subunit molecular weight (32 000) to the enzymes previously isolated from rat and mouse. The bovine liver enzyme immunologically crossreacted withS-adenosylmethionine decarboxylase from resting and mitogenically activated bovine lymphocytes. The rate of enzyme synthesis in activated lymphocytes was determined by labeling the cells with [3H]leucine and isolating the radioactive decarboxylase by affinity chromatography and sodium dodecyl sulfate gel electrophoresis. The rate of enzyme syntheis was increased 10-fold by 9 h after mitogen treatment, which accounts for the initial increase in cellular enzymatic. There was no further incraese in the rate ofS-adenosylmethionine decarboxylase synthesis that correlated with a second elevation of activity occuring at approx. 24 h after mitogenic activation. It was concluded that the second increase in enzyme activity was due to lengthening the intracellular half-life of the enzyme by 2-fold.