Selection of Best Indicators for Ranking and Determination of Bottleneck Enzymes in Metabolic Reaction Systems
Selection of Best Indicators for Ranking and Determination of Bottleneck Enzymes in Metabolic Reaction Systems
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DOI：
10.1021/ie100911h
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发表时间：
2010-10-20
影响因子：
4.2
通讯作者：
Shiraishi, Fumihide
中科院分区：
文献类型：
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作者：
Sriyudthsak, Kansuporn;Shiraishi, Fumihide
动态对数增益及其修正在理论上可以用作代谢反应系统中的瓶颈排序（BR）指标。然而，这是不够明确，他们是否可以成功地用于实际应用。因此，目前的工作重点是选择最佳的BR指标，在瞬时和整体情况。一个改进的乙醇发酵模型被用作案例研究。结果表明，动态对数增益与给定时间的所需产物浓度的数学乘积是最好的瞬时BR指标，而其在发酵过程结束时的值是最好的总体BR指标。前者可用于观察所需产品浓度的时间进程并确定待终止的工艺时间。后者用于对瓶颈酶进行排序，以确定应改变哪种酶的活性以获得更高的所需产物浓度。此外，讨论了利用总BR指标来预测最终所需产物浓度增加多少。
Dynamic logarithmic gain and its modifications can be theoretically used as a bottleneck ranking (BR) indicator in a metabolic reaction system. However, it is not sufficiently explicit whether they can be successfully used in practical applications. The present work therefore focuses on the selection of best BR indicators in both instantaneous and overall cases. A modified ethanol fermentation model is used as a case study. The results indicate that the mathematical product of dynamic logarithmic gain and desired product concentration at a given time is the best instantaneous BR indicator, whereas its value at the end time of the fermentation process is the best overall BR indicator. The former is useful for observing the time course of the desired product concentration and determining a process time to be terminated. The latter is for ranking bottleneck enzymes to determine which enzyme activity should be changed to attain a higher desired product concentration. Moreover, discussion is made on the utilization of the overall BR indicators to predict how much the final desired product concentration is increased.