Identification of antigenic peptides from novel renal cancer stem-like cell antigen, DNAJB8
Identification of antigenic peptides from novel renal cancer stem-like cell antigen, DNAJB8
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新型肾癌干细胞样细胞抗原 DNAJB8 中抗原肽的鉴定
DOI：
10.1016/j.bbrc.2017.10.146
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Hara I
中科院分区：
文献类型：
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作者：
Nishizawa S;Hirohashi Y;Kusumoto H;Wakamiya T;Iguchi T;Yamashita S;Iba A;Kikkawa K;Kohjimoto Y;Torigoe T;Hara I
目的鉴定肿瘤干细胞（cancer stem-样细胞，CSCs）抗原DNAJB8的抗原多肽，建立小鼠CSCs靶向免疫治疗模型。材料和方法为了诱导dnajb8特异性免疫反应，我们用三种候选HLA-A*24:02限制性肽鸡尾酒刺激抗原呈递细胞刺激人CD8+淋巴细胞，并评估肽特异性人细胞毒性T淋巴细胞（ctl）的诱导作用。其中一个抗原肽的氨基酸序列与相应的小鼠DNAJB8相同。我们在小鼠模型上评价了肽免疫对CTL的诱导作用。结果证实了肽特异性干扰素γ分泌和诱导的人ctl的细胞毒活性。用该肽对小鼠进行体内免疫，可在小鼠CD8+T细胞中观察到肽特异性CTL反应。此外，与阴性对照相比，该肽免疫具有显著的抗肿瘤作用。结论dnajb8衍生肽是一种新的cscs靶向免疫治疗候选肽，可用于小鼠模型评价cscs靶向免疫治疗。
ObjectivesTo identify antigenic peptides of cancer stem-like cells (CSCs) antigen, DNAJB8, and establish a mouse CSCs-targeting immunotherapy model.Materials and methodsTo induce DNAJB8-specific immune reaction, we stimulated human CD8+lymphocytes with antigen-presenting cells pulsed with a cocktail of three candidate HLA-A*24:02 restricted peptides and assessed peptide specific human cytotoxic T lymphocytes (CTLs) induction. One of the antigenic peptides showed identical amino acid sequence as corresponding mouse DNAJB8. We evaluated CTL induction with the peptide immunization in mouse model.ResultsWe confirmed peptide-specific interferon-γ secretions and cytotoxic activities of induced human CTLs. In vivo immunization with the peptide to mice, peptide-specific CTL response could be observed in mouse CD8+T cells. Furthermore, immunization with the peptide showed significant anti-tumor effects compared with negative controls.ConclusionDNAJB8-derived peptide is a novel candidate for CSCs-targeting immunotherapy, and mouse models can be used to evaluate CSCs-targeting immunotherapy.