Rapid contrast evaluation method based on affinity beads and backscattered electron imaging for the screening of electron stains.
Rapid contrast evaluation method based on affinity beads and backscattered electron imaging for the screening of electron stains.
复制标题
基于亲和珠和背散射电子成像的快速对比度评估方法，用于筛选电子染色剂。
作者：
Kaku;H.;Inoue;K.;Muranaka;Y.;Park;P.;Ikeda;K.
铀酰盐具有毒性和放射性;因此，已经进行了几项研究来筛选电子染色剂的替代品。在这方面，对比度评估过程是耗时的，并且所获得的结果是不一致的。在这项研究中，我们开发了一种新的对比度评价方法，使用亲和珠和背散射电子图像（BSEI），使用扫描电子显微镜获得。BSEI在每个电子染色处理的对比度与透射电镜图像的对比度相关。亲和珠独立地与细胞组分结合。蛋白质和DNA样品增强了电子染色处理的图像对比度，然而，这是没有观察到的糖。当用铅双重染色时，蛋白质缀合珠显示图像对比度的加和效应。然而，在DNA缀合的珠中未观察到双重染色的累加效应。不同的化学性质的寡肽显示不同的图像对比度时，处理与每个电子染色。这种基于BSEI的评估方法不仅能够筛选替代电子染色剂，而且还有助于分析细胞结构电子染色的潜在机制。
Uranyl salts are toxic and radioactive; therefore, several studies have been conducted to screen for substitutes of electron stains. In this regard, the contrast evaluation process is time consuming and the results obtained are inconsistent. In this study, we developed a novel contrast evaluation method using affinity beads and a backscattered electron image (BSEI), obtained using scanning electron microscopy. The contrast ratios of BSEI in each electron stain treatment were correlated with those of transmission electron microscopic images. The affinity beads bound to cell components independently. Protein and DNA samples were enhanced by image contrast treated with electron stains; however, this was not observed for sugars. Protein-conjugated beads showed an additive effect of image contrast when double-stained with lead. However, additive effect of double staining was not observed in DNA-conjugated beads. The varying chemical properties of oligopeptides showed differences in image contrast when treated with each electron stain. This BSEI-based evaluation method not only enables screening for alternate electron stains, but also helps analyze the underlying mechanisms of electron staining of cellular structures.