ACTION OF 5-THIO-D-GLUCOSE AND ITS 1-PHOSPHATE WITH HEXOKINASE AND PHOSPHOGLUCOMUTASE
ACTION OF 5-THIO-D-GLUCOSE AND ITS 1-PHOSPHATE WITH HEXOKINASE AND PHOSPHOGLUCOMUTASE
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DOI：
10.1016/0003-9861(75)90180-0
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发表时间：
1975-01-01
影响因子：
3.9
通讯作者：
WHISTLER, RL
中科院分区：
文献类型：
--
作者：
CHEN, M;WHISTLER, RL
该实验室之前的工作表明，5-硫代-d-葡萄糖是 d-葡萄糖主动转运的竞争性抑制剂。目前的工作表明，硫代糖类似物及其1-磷酸盐也可以干扰d-葡萄糖6-P的形成。5-硫代-d-葡萄糖作为酵母己糖激酶的底物，其Km为4mm，V为8.8nmol/min/μg蛋白质。该类似物竞争性抑制 d-葡萄糖磷酸化，Ki 为 20 mm。5-硫代-d-葡萄糖 1-P 可作为兔骨骼肌磷酸葡萄糖变位酶的底物，Km 为 60 μm，V 为 0.17 μmol/min/μg 蛋白质。因此，5-硫代-d-葡萄糖 1-P 的行为类似于 d-葡萄糖 1-P 的代谢类似物。 5-Thio-d-glucose 1-P 是 d-glucose 1-P 转化为 6-P 的竞争性抑制剂，aKiof 16.2 μm。通过 5-thio-d-glucose 磷酸化和 5-thio-d-glucose 1-P 转化产生的 5-Thio-d-glucose 6-P 通过色谱迁移率和颜色反应进行鉴定。
Previous work from this laboratory has shown that 5-thio-d-glucose is a competitive inhibitor for active transport ofd-glucose. The present work indicates that the thiosugar analog and its 1-phosphate can also interfere withd-glucose 6-P formation.5-Thio-d-glucose serves as a substrate for yeast hexokinase with aKmof 4 mm, andVof 8.8 nmol/min/μg of protein. The analog competitively inhibitsd-glucose phosphorylation with aKiof 20 mm.5-Thio-d-glucose 1-P can act as a substrate for rabbit skeletal muscle phosphoglucomutase with aKmof 60 μmandVof 0.17 μmol/min/μg of protein. Thus, 5-thio-d-glucose 1-P behaves as a near metabolic analog ofd-glucose 1-P. 5-Thio-d-glucose 1-P is a competitive inhibitor ofd-glucose 1-P conversion to the 6-P with aKiof 16.2 μm.5-Thio-d-glucose 6-P produced by phosphorylation of 5-thio-d-glucose and by conversion from 5-thio-d-glucose 1-P was identified by chromatographic mobility and by color reactions.