Expression of the endothelial cell differentiation gene 7 (EDG-7), a lysophosphatidic acid receptor, in ovarian tumor
Expression of the endothelial cell differentiation gene 7 (EDG-7), a lysophosphatidic acid receptor, in ovarian tumor
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DOI：
10.1111/j.1447-0756.2005.00299.x
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发表时间：
2005-01-01
影响因子：
1.6
通讯作者：
Kanzaki, H
中科院分区：
文献类型：
--
作者：
Nakamoto, T;Yasuda, K;Kanzaki, H
目的：溶血磷脂酸（LPA）作为促有丝分裂剂受到关注，因为生理活性脂质通过与特异性受体（内皮细胞分化基因（EDG）家族）相互作用刺激卵巢癌细胞生长。在本研究中，我们研究了EDG-7 mRNA的表达，EDG家族的一部分，在两个人卵巢癌和建立人卵巢癌细胞株。方法：RNA提取从6个卵巢癌细胞系和多个癌组织和正常卵巢组织。EDG-7 mRNA的表达采用逆转录-聚合酶链反应和北方印迹法进行测定，使用还原型甘油二酸脱氢酶和S26作为内部controls.Results：在测试的细胞系中，EDG-7 mRNA在CRL-11731和CRL-1572中表达最强，在CRL-11732中表达较低，但仍相当高。EDG-7 mRNA在MCAS、CRL-11730和TYKnu中的表达有限。在卵巢癌组织中，EDG-7 mRNA在卵巢样腺癌和浆液性囊腺癌中表达最高。EDG-7 mRNA在透明细胞腺癌中的表达有限，在粘液性囊腺癌中未检测到。结论：EDG-7在卵巢癌中的强表达提示LPA和EDG-7（一种LPA受体）之间的关系参与了某些组织学亚型卵巢癌细胞的生长和增殖。
Aim: Lysophosphatidic acid (LPA) has received attention as a mitogen because the physiologically active lipid stimulates ovarian cancer cell growth by interacting with specific receptors, the endothelial cell differentiation gene (EDG) family. In the present study, we have investigated the expression of EDG-7 mRNA, part of the EDG family, in both human ovarian cancers and established human ovarian cancer cell lines.Methods: RNA was extracted from six ovarian cancer cell lines and multiple cancerous and normal ovarian tissues. The expression of EDG-7 mRNA was measured using reverse transcription-polymerase chain reaction and northern blotting, using reduced glyceraldehyde-phosphate dehydrogenase and S26 as internal controls.Results: Of the cell lines tested, EDG-7 mRNA was expressed most intensely in CRL-11731 and CRL-1572 and at a lesser but still substantial level in CRL-11732. The expression of EDG-7 mRNA was limited in MCAS, CRL-11730 and TYKnu. In the ovarian cancer tissues, EDG-7 mRNA was expressed most highly in endometrioid adenocarcinoma and serous cystadenocarcinoma. The expression of EDG-7 mRNA was limited in clear cell adenocarcinoma and undetectable in mucinous cystadenocarcinoma.Conclusions: The intense EDG-7 expression in ovarian cancers suggests that the relation between LPA and EDG-7 (an LPA receptor) is involved in cancer cell growth and proliferation in some histologic subtypes of ovarian cancer.