WIND1 induces dynamic metabolomic reprogramming during regeneration in Brassica napus
WIND1 induces dynamic metabolomic reprogramming during regeneration in Brassica napus
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DOI：
10.1016/j.ydbio.2018.07.006
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发表时间：
2018-10-01
影响因子：
2.7
通讯作者：
Sugimoto, Keiko
中科院分区：
文献类型：
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作者：
Iwase, Akira;Mita, Kento;Sugimoto, Keiko
植物在胁迫条件下通常表现出较高的再生能力。响应于各种类型的应激而产生的信号作为从头器官发生的关键触发器，但是这些信号分子的身份在很大程度上是未知的。我们以前确定了一个AP 2/ERF转录因子，伤口诱导的去分化1（WIND 1），作为一个关键的调节参与创伤诱导的细胞重编程在拟南芥。本研究以甘蓝型油菜（B. napus）增强愈伤组织形成和随后的器官再生。基因表达分析显示AtWIND 1增强了B的表达。napus同源物的增强子芽再生/DRN（ESR 1/DRN），其是拟南芥中WIND 1的直接靶标。此外，时程激素分析表明，改变内源性生长素/细胞分裂素的平衡存在于AtWIND 1激活的B。油菜外植体。此外，我们的质谱分析揭示了AtWIND 1激活的外植体中的动态代谢组学重编程，包括几种化合物的积累，例如脯氨酸，γ-氨基丁酸（GABA）和腐胺，这些化合物在历史上被用作添加剂以增强组织培养中的植物细胞重编程。因此，我们的研究结果为WIND 1如何促进细胞重编程提供了新的见解。
Plants often display a high competence for regeneration under stress conditions. Signals produced in response to various types of stress serve as critical triggers for de novo organogenesis, but the identity of these signaling molecules underlying cellular reprogramming are largely unknown. We previously identified an AP2/ERF transcription factor, WOUND INDUCED DEDIFFERENTIATION1 (WIND1), as a key regulator involved in wound-induced cellular reprogramming in Arabidopsis. In this study, we found that activation of Arabidopsis WIND1 (AtWIND1) in hypocotyl explants of Brassica napus (B. napus) enhances callus formation and subsequent organ regeneration. Gene expression analyses revealed that AtWIND1 enhances expression of B. napus homologs of ENHANCER OF SHOOT REGENERATIONI/DORNROSCHEN (ESR1/DRN), which is a direct target of WIND1 in Arabidopsis. Further, time-course hormonal analyses showed that an altered balance of endogenous auxin/cytokinin exists in AtWIND1-activated B. napus explants. Our mass spectrometry analyses, in addition, uncovered dynamic metabolomic reprogramming in AtWIND1-activated explants, including accumulation of several compounds, e.g. proline, gamma aminobutyric acid (GABA), and putrescine, that have historically been utilized as additives to enhance plant cell reprogramming in tissue culture. Our findings thus provide new insights into how WIND1 functions to promote cell reprogramming.