Human erythropoietin receptor: cloning, expression, and biologic characterization.
Human erythropoietin receptor: cloning, expression, and biologic characterization.
复制标题
DOI：
10.1182/blood.v76.1.31.bloodjournal76131
复制
发表时间：
1990-07
期刊：
影响因子：
20.3
通讯作者：
S. Jones;Alan D. D’Andrea;Lora L. Haines;Gordon G. Wong
中科院分区：
文献类型：
--
作者：
S. Jones;Alan D. D’Andrea;Lora L. Haines;Gordon G. Wong
我们已经从红白血病细胞系OCIM 1和胎肝中分离出了鼠促红细胞生成素受体（mEPO-R）的人类同源物。该受体的cDNA和蛋白质序列与mEPO-R的同源性均为82%。人cDNA在COS细胞中的异源表达产生约66 Kd的蛋白质。该蛋白质可以与针对mEPO-R的氨基末端产生的抗体或与结合其受体的EPO结合的单克隆抗体特异性免疫沉淀。将放射性碘标记的EPO与表达人EPO-R的COS细胞交联，得到受体的表观分子量为66和100 Kd。鼠白细胞介素-3依赖性前B淋巴细胞系Ba/F3通过将人cDNA转染到细胞中并选择在含EPO的培养基中生长而成为EPO依赖性的。
We have isolated the human homologue of the murine erythropoietin receptor (mEPO-R) from an erythroleukemia line, OCIM1, and from fetal liver. Both the cDNA and protein sequence of the human receptor were 82% homologous to the mEPO-R. Heterologous expression of the human cDNA in COS cells yielded a protein of about 66 Kd. The protein could be specifically immunoprecipitated with either an antibody raised against the amino terminus of mEPO-R or by a monoclonal antibody that bound EPO bound to its receptor. Cross-linking of radioiodinated EPO to COS cells expressing the human EPO-R gave apparent molecular weights of 66 and 100 Kd for the receptor. The murine interleukin-3-dependent pre-B-lymphocyte cell line, Ba/F3, was made EPO-dependent by transfection of the human cDNA into the cells and selecting for growth in EPO-containing media.