Heterocyst patterns without patterning proteins in cyanobacterial filaments
Heterocyst patterns without patterning proteins in cyanobacterial filaments
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DOI：
10.1016/j.ydbio.2007.09.045
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发表时间：
2007-12-01
影响因子：
2.7
通讯作者：
Rutenberg, Andrew D.
中科院分区：
文献类型：
--
作者：
Allard, Jun F.;Hill, Alison L.;Rutenberg, Andrew D.
我们已经定量模拟异形胞分化后，固定的氮步下降的丝状蓝藻鱼腥藻属PCC 7120没有横向抑制由于图案蛋白Pats或HetN。我们使用细胞生长和分裂连同固定氮动力学，并允许异形胞分化后，当地可用的固定氮耗尽。固定氮沿着共享周质空间的缓慢运输允许快速生长的细胞先于它们的邻居分化。细胞间生长速率的差异决定了最初的异形胞模式。早期释放的固定氮从定向异形胞允许营养细胞的显着部分被保留在以后的时间。我们恢复了Khudyakov和Golden [Khudyakov，I.Y.，戈尔登，J.W.，2004. HetR是鱼腥藻PCC 7120中异形胞分化的主要调节因子，其不同功能可以通过突变来分离。Proc. Nail. Acad. Sci.联合S. A. 101，16040-16045]。(c)2007年爱思唯尔公司All rights reserved.
We have quantitatively modeled heterocyst differentiation after fixed nitrogen step-down in the filamentous cyanobacterium Anabaena sp. PCC 7120 without lateral inhibition due to the patterning proteins Pats or HetN. We use cell growth and division together with fixed-nitrogen dynamics and allow heterocysts to differentiate upon the local exhaustion of available fixed nitrogen. Slow transport of fixed nitrogen along a shared periplasmic space allows for fast growing cells to differentiate ahead of their neighbors. Cell-to-cell variability in growth rate determines the initial heterocyst pattern. Early release of fixed nitrogen from committed heterocysts allows a significant fraction of vegetative cells to be retained at later times. We recover the experimental heterocyst spacing distributions and cluster size distributions of Khudyakov and Golden [Khudyakov, I.Y., Golden, J.W., 2004. Different functions of HetR, a master regulator of heterocyst differentiation in Anabaena sp PCC 7120, can be separated by mutation. Proc. Nail. Acad. Sci. U. S. A. 101, 16040-16045]. (c) 2007 Elsevier Inc. All rights reserved.