Ganglioside GD3 monoclonal antibody-induced paxillin tyrosine phosphorylation and filamentous actin assembly in cerebellar growth cones.
Ganglioside GD3 monoclonal antibody-induced paxillin tyrosine phosphorylation and filamentous actin assembly in cerebellar growth cones.
复制标题
神经节苷脂 GD3 单克隆抗体诱导小脑生长锥中桩蛋白酪氨酸磷酸化和丝状肌动蛋白组装。
DOI：
10.1111/j.1471-4159.2010.07071.x
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Kasahara K
中科院分区：
文献类型：
--
作者：
Yuyama K;Sekino-Suzuki N;Yamamoto N;Kasahara K
神经化学杂志。（2011）116，845- 850.摘要我们已经证明，神经节苷脂GD 3（R24）的抗体免疫沉淀来自原代小脑颗粒细胞的src家族酪氨酸激酶林恩，并且完整细胞的R24处理诱导林恩活化和几种底物的快速酪氨酸磷酸化，表明神经节苷脂GD 3与林恩的功能关联。在这项研究中，R24处理原代小脑颗粒细胞增强了酪氨酸残基118处桩蛋白的磷酸化，并诱导丝状肌动蛋白组装和神经突生长。小脑生长锥膜组分的R24处理诱导68 kDa蛋白的显著酪氨酸磷酸化，该蛋白在酪氨酸残基118处与磷酸轴素共迁移。 已知桩蛋白的酪氨酸磷酸化调节细胞形态中的肌动蛋白细胞骨架依赖性变化。神经节苷脂GD 3的信号转导通过桩蛋白的酪氨酸磷酸化参与生长锥形态。
J. Neurochem.(2011)116, 845–850.AbstractWe have demonstrated that antibody to ganglioside GD3 (R24) immunoprecipitates src‐family tyrosine kinase Lyn from primary cerebellar granule cells and R24 treatment of the intact cells induces Lyn activation and rapid tyrosine phosphorylation of several substrates, suggesting the functional association of ganglioside GD3 with Lyn. In this study, R24 treatment of primary cerebellar granule cells enhances phosphorylation of paxillin at tyrosine residue 118 and induces filamentous actin assembly and neurite outgrowth. R24 treatment of cerebellar growth cone membrane fraction induces prominent tyrosine phosphorylation of 68 kDa protein which comigrates with phosphopaxillin at tyrosine residue 118. Tyrosine phosphorylation of paxillin is known to regulate actin cytoskeleton‐dependent changes in cell morphology. Signal transduction by ganglioside GD3 is involved in growth cone morphology via tyrosine phosphorylation of paxillin.