Stochastic regulation of cell migration from the efferent lymph to oxazolone-stimulated skin
Stochastic regulation of cell migration from the efferent lymph to oxazolone-stimulated skin
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DOI：
10.4049/jimmunol.166.3.1517
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发表时间：
2001-02-01
影响因子：
4.4
通讯作者：
Mentzer, SJ
中科院分区：
文献类型：
--
作者：
West, CA;He, C;Mentzer, SJ
全身免疫反应是一个动态的过程，涉及淋巴细胞从抗原刺激的淋巴结运到周围组织。在绵羊身上的研究表明，在Ag刺激后，传出淋巴中有几个阶段的细胞输出。当皮肤接触性致敏剂被用作Ag时，传出淋巴细胞输出的峰值类似于Ag刺激后96h，并与细胞进入皮肤的时间相关。为了研究这一高输出期淋巴细胞在皮肤淋巴细胞性炎症中的相对作用，我们用常用的接触性增敏剂2-苯基-4-乙氧亚甲基-5-恶唑酮(恶唑酮)刺激成年绵羊的皮肤和引流肩前淋巴结。在整个实验期间，引流Ag刺激和对侧对照淋巴结的传出淋巴管被置入管内。用荧光示踪剂对在最大浸润期前72小时内离开淋巴结的淋巴细胞进行差示标记，重新注入动脉循环，并追踪到Ag刺激的部位。注射结束时(Ag刺激后96h)的皮肤定量组织细胞术显示，来自Ag刺激的淋巴结的移行细胞比对侧对照多(中位数18.5vs15.5个/视野；p<；0.05)。然而，在校正了淋巴结的总细胞输出量后，银刺激的迁移细胞在皮肤中的比例是对侧对照细胞的3.8倍。这些结果表明，原位免疫反应通常反映了外周血液中可招募淋巴细胞的频率。
The systemic immune response is a dynamic process involving the trafficking of lymphocytes from the Ag-stimulated lymph node to the peripheral tissue. Studies in sheep have demonstrated several phases of cell output in the efferent lymph after Ag stimulation. When skin contact sensitizers are used as Ag, the efferent lymph cell output peaks similar to 96 h after Ag stimulation and is temporally associated with the recruitment of cells into the skin. To investigate the relative contribution of this high-output phase of efferent lymphocytes to lymphocytic inflammation in the skin, we used a common contact sensitizer 2-phenyl-4-ethoxymethylene-5-oxazolone (oxazolone) to stimulate the skin and draining prescapular lymph node of adult sheep. The efferent lymph ducts draining the Ag-stimulated and contralateral control lymph nodes were cannulated throughout the experimental period. The lymphocytes leaving the lymph nodes during the 72-h period before maximum infiltration were differentially labeled with fluorescent tracers, reinjected into the arterial circulation, and tracked to the site of Ag stimulation. Quantitative tissue cytometry of the skin at the conclusion of the injection period (96 h after Ag stimulation) demonstrated more migratory cells derived from the Ag-stimulated lymph node than the contralateral control (median 18.5 vs 15.5 per field; p < 0.05). However, when corrected for total cell output of the lymph node, the Ag-stimulated migratory cells were 3.8-fold more prevalent in the skin than the contralateral control cells. These results suggest that the in situ immune response generally mirrors the frequency of recruitable lymphocytes in the peripheral blood.