MicroRNA-124 is a subventricular zone neuronal fate determinant.
MicroRNA-124 is a subventricular zone neuronal fate determinant.
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DOI：
10.1523/jneurosci.0558-12.2012
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发表时间：
2012-06-27
期刊：
影响因子：
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通讯作者：
Jakobsson J
中科院分区：
文献类型：
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作者：
Åkerblom M;Sachdeva R;Barde I;Verp S;Gentner B;Trono D;Jakobsson J
新的神经元是从具有星形胶质细胞特性的神经干细胞中不断产生的，这些神经干细胞在出生后和成人大脑中非常接近心室。在这项研究中，我们发现microRNA-124（miR-124）决定了小鼠脑室下区的出生后神经发生。使用转基因报告小鼠，我们表明，miR-124的表达开始在快速扩增的祖细胞，并保持在所得的神经元中表达。当我们在体内稳定抑制miR-124时，神经发生被阻断，导致嗅球中出现具有星形胶质细胞特征的异位细胞。相反，当我们过表达miR-124时，神经干细胞不能维持在脑室下区，神经发生丧失。总之，我们的研究结果表明，miR-124是一个神经元的命运决定因素在脑室下区。
New neurons are continuously generated from neural stem cells with astrocyte properties, which reside in close proximity to the ventricle in the postnatal and adult brain. In this study we found that microRNA-124 (miR-124) dictates postnatal neurogenesis in the mouse subventricular zone. Using a transgenic reporter mouse we show that miR-124 expression is initiated in the rapid amplifying progenitors and remains expressed in the resulting neurons. When we stably inhibited miR-124 in vivo, neurogenesis was blocked, leading to the appearance of ectopic cells with astrocyte characteristics in the olfactory bulb. Conversely, when we overexpressed miR-124, neural stem cells were not maintained in the subventricular zone and neurogenesis was lost. In summary, our results demonstrate that miR-124 is a neuronal fate determinant in the subventricular zone.