Total flavonoids from celery suppresses RANKL-induced osteoclast differentiation and bone resorption function via attenuating NF-κB and p38 pathways in RAW264.7 cells
Total flavonoids from celery suppresses RANKL-induced osteoclast differentiation and bone resorption function via attenuating NF-κB and p38 pathways in RAW264.7 cells
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芹菜中的总黄酮通过减弱 RAW264.7 细胞中的 NF-kappa B 和 p38 通路来抑制 RANKL 诱导的破骨细胞分化和骨吸收功能
DOI：
10.1016/j.jff.2020.103949
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发表时间：
2020-06-01
影响因子：
5.6
通讯作者：
Liu, Xin
中科院分区：
文献类型：
--
作者：
Jia, Lujuan;Shi, Liying;Liu, Xin
破骨细胞过度活化引起的骨骼疾病严重影响老年人的健康。因此，抑制破骨细胞的过度活化成为治疗骨质疏松症的有效策略。芹菜是我国传统的大众化蔬菜，营养价值高，富含黄酮类化合物，具有多种药理活性。本研究旨在观察芹菜总黄酮（TFC）对破骨细胞生成的药理作用，并探讨其分子机制。结果表明，TFC可抑制破骨细胞分化和骨吸收，且无明显毒副作用。Western blot分析和qPCR结果表明，TFC可抑制I kappa B α的降解、NF-κ B B和p38的磷酸化，从而降低破骨细胞特异性基因的表达水平。本研究结果可能对TFC作为功能性食品成分的应用产生积极影响，对破骨细胞相关疾病具有潜在的预防和治疗作用。
Skeletal diseases caused by excessive activation of osteoclasts seriously affect elderly's health. Therefore, inhibiting excessive activation of osteoclasts become effective therapeutic strategy for osteoporosis. Celery, a traditional and popular Chinese vegetable with high nutritional value, is rich in flavonoids which have a variety of pharmacological activities. The present study aimed to determine the pharmacological effect of total flavonoids of celery (TFC) on osteoclastogenesis, and to explore the molecular mechanisms. Results showed that TFC inhibited osteoclast differentiation and bone resorption without significant toxic and side effect. Furthermore, the results of western blot analysis and qPCR indicated that TFC inhibited the degradation of I kappa B alpha, phosphorylation of NF-kappa B and p38, and subsequently attenuated the expression levels of osteoclast-specific genes. The results of this study may have a positive impact on the application of TFC as functional food ingredients, which has potential preventive and therapeutic effects on osteoclast-related diseases.