Transcriptional modulation of transin gene expression by epidermal growth factor and transforming growth factor beta.
Transcriptional modulation of transin gene expression by epidermal growth factor and transforming growth factor beta.
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表皮生长因子和转化生长因子β对反式蛋白基因表达的转录调节。
DOI：
10.1128/mcb.8.6.2479-2483.1988
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发表时间：
1988
影响因子：
5.3
通讯作者：
Magun,BE
中科院分区：
文献类型：
--
作者：
Machida,CM;Muldoon,LL;Rodland,KD;Magun,BE
Transin是一种转化相关基因，在多种癌基因转化的大鼠成纤维细胞和恶性小鼠皮肤癌中组成性表达，但在良性乳头状瘤或正常皮肤中不表达。已经证明，在未转化的Rat-1细胞中，transin RNA表达受表皮生长因子（EGF）的正向调节，受转化生长因子β（TGF-β）的负向调节;发现其他肽类生长因子对transin表达无影响。这里提出的结果表明，蛋白质的合成和EGF受体的持续占用EGF所需的持续诱导transin RNA。用TGF-β处理抑制EGF诱导transin的能力，无论是在转录水平上通过核连续分析测定还是在transin RNA积累水平上通过细胞RNA的北方（RNA）印迹分析测定。TGF-β既阻断了EGF对transin转录的初始诱导，又在长期治疗期间停止了transin转录本的产生。这些结果表明，TGF-β在转录水平上拮抗EGF介导的transin基因表达的诱导。
Transin is a transformation-associated gene which is expressed constitutively in rat fibroblasts transformed by a variety of oncogenes and in malignant mouse skin carcinomas but not benign papillomas or normal skin. It has been demonstrated that, in nontransformed Rat-1 cells, transin RNA expression is modulated positively by epidermal growth factor (EGF) and negatively by transforming growth factor β (TGF-β); other peptide growth factors were found to have no effect on transin expression. Results presented here indicate that both protein synthesis and continuous occupancy of the EGF receptor by EGF were required for sustained induction of transin RNA. Treatment with TGF-β inhibited the ability of EGF to induce transin, whether assayed at the transcriptional level by nuclear run-on analysis or at the level of transin RNA accumulation by Northern (RNA) blot analysis of cellular RNA. TGF-β both blocked initial induction of transin transcription by EGF and halted established production of transin transcripts during prolonged treatment. These results suggest that TGF-β acts at the transcriptional level to antagonize EGF-mediated induction of transin gene expression.