AN ANALYSIS OF 5'-NONCODING SEQUENCES FROM 699 VERTEBRATE MESSENGER-RNAS
AN ANALYSIS OF 5'-NONCODING SEQUENCES FROM 699 VERTEBRATE MESSENGER-RNAS
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DOI：
10.1093/nar/15.20.8125
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发表时间：
1987-10-26
影响因子：
14.9
通讯作者：
KOZAK, M
中科院分区：
文献类型：
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作者：
KOZAK, M
从699个脊椎动物的mRNAs中编制了5‘-非编码序列。(GCC)GCCAGCCATGG是脊椎动物翻译起始的共识序列。该基序中最保守的位置是位置-3的嘌呤(ATG密码子上游的三个核苷酸)；97%的脊椎动物mRNA在该位置有嘌呤，最常见的是A-G的周期性出现(位置−3，−6，−9)。上游ATG密码子出现在不到10%的脊椎动物mRNAs中；一个明显的例外是癌基因转录本，其中三分之二的转录本在主要开放阅读框架开始之前具有ATG密码子。大多数脊椎动物mRNAs的前导序列大小在20到100个核苷酸之间。讨论了较短和较长5‘-非编码序列的意义。
5'-Noncoding sequences have been compiled from 699 vertebrate mRNAs. (GCC)GCCAGCCATGG emerges as the consensus sequence for initiation of translation in vertebrates. The most highly conserved position in that motif is the purine in position -3 (three nucleotides upstream from the ATG codon); 97% of vertebrate mRNAs have a purine, most often A, in that position- The periodical occurrence of G (in positions −3, −6, −9) is discussed. Upstream ATG codons occur in fewer than 10% of vertebrate mRNAs-at-large; a notable exception are oncogene transcripts, two-thirds of which have ATG codons preceding the start of the major open reading frame. The leader sequences of most vertebrate mRNAs fall in the size range of 20 to 100 nucleotides. The significance of shorter and longer 5' -noncoding sequences is discussed.