Application of cysteamine-capped gold nanoparticles for early detection of lung cancer-specific miRNA (miR-25) in human blood plasma
Application of cysteamine-capped gold nanoparticles for early detection of lung cancer-specific miRNA (miR-25) in human blood plasma
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DOI：
10.1039/c7ay01098b
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发表时间：
2017-07-07
影响因子：
3.1
通讯作者：
Zare, Hamid R.
中科院分区：
文献类型：
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作者：
Asadzadeh-Firouzabadi, Amineh;Zare, Hamid R.
在这项研究中，我们设计了一种基于半胱胺覆盖的金纳米颗粒（Cys-AuNPs）的纳米基因传感器，使其能够检测与肺癌相关的miRNA（miR-25）。用戊二醛（GA）将单链探针（ssprobe）共价固定在Cys-AuNPs/玻碳电极（GCE）表面，并与肺癌特异性miRNA杂交。采用扫描电镜（SEM）、循环伏安（CV）和电化学阻抗谱（EIS）等方法对该传感器的制备过程进行了表征。利用电化学阻抗谱研究了该纳米传感器对miR-25的检测性能。在最佳条件下，所提出的纳米基因传感器被证明具有1.0 x 10（-12）至1.0 x 10（-10）M和1.0 x 10（-10）至1.0 x 10（-6）M的两个miR-25浓度范围，检测限为2.5 x 10（-13）M。此外，它可以显着区分完全匹配的miRNA链和包含单碱基突变的miRNA链。此外，这种纳米基因传感器能够在不使用PCR或任何其他扩增方法的情况下检测人血浆中的miR-25。
In this study, we designed a nanogenosensor based on cysteamine-capped gold nanoparticles (Cys-AuNPs) that makes it possible to detect the miRNA (miR-25) related to lung cancer. A single stranded probe (ssprobe) was covalently immobilized on the surface of a Cys-AuNPs/glassy carbon electrode (GCE) by glutaraldehyde (GA), and then the ss-probe was hybridized with the lung cancer-specific miRNA. The preparation process of the proposed electrochemical nanogenosensor was characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). Using EIS, the analytical performance of this nanogenosensor in detecting miR-25 was investigated. Under optimal conditions, the proposed nanogenosensor proved to have two miR-25 concentration ranges of 1.0 x 10(-12) to 1.0 x 10(-10) M and 1.0 x 10(-10) to 1.0 x 10(-6) M and a limit of detection of 2.5 x 10(-13) M. Also, it could significantly make a distinction between a fully matched miRNA strand and a miRNA strand comprising a single-base mutation. Furthermore, this nanogenosensor is capable of detecting miR-25 in human blood plasma without using PCR or any other amplification methods.