Ultrasensitive rapid detection of human serum antibody biomarkers by biomarker-capturing viral nanofibers.
Ultrasensitive rapid detection of human serum antibody biomarkers by biomarker-capturing viral nanofibers.
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通过捕获生物标记物的病毒纳米纤维超灵敏快速检测人血清抗体生物标记物。
DOI：
10.1021/acsnano.5b01074
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发表时间：
2015
期刊：
影响因子：
17.1
通讯作者：
Mao C
中科院分区：
文献类型：
--
作者：
Wang Y;Ju Z;Cao B;Gao X;Zhu Y;Qiu P;Xu H;Pan P;Bao H;Wang L;Mao C
白色念珠菌（C. albicans）感染会导致癌症患者的高死亡率。由于现有诊断系统的灵敏度较低，因此需要一种新的灵敏检测方法来对其进行诊断。为此，我们利用基因展示两种功能肽的能力，一种负责识别癌症患者血清中感染的生物标志物（抗分泌天冬氨酰蛋白酶2 IgG抗体），另一种负责在单个丝状fd噬菌体（一种对人类安全的细菌特异性病毒）上结合磁性纳米粒子（MNP）。所得噬菌体首先用 MNP 修饰，然后从血清中捕获生物标志物。然后对噬菌体结合的生物标志物进行磁性富集和生化检测。该方法大大提高了生物标志物检测的灵敏度和特异性。每个血清样本的平均检测时间仅约6小时，远短于临床使用的金标准方法约1周的时间。我们的纳米生物技术方法的检测限约为1.1 pg/mL，比传统的基于抗原的方法低约2个数量级，为基于病毒的疾病诊断开辟了新途径。
Candida albicans (C. albicans) infection causes high mortality rates within cancer patients. Due to the low sensitivity of the current diagnosis systems, a new sensitive detection method is needed for its diagnosis. Toward this end, here we exploited the capability of genetically displaying two functional peptides, one responsible for recognizing the biomarker for the infection (antisecreted aspartyl proteinase 2 IgG antibody) in the sera of cancer patients and another for binding magnetic nanoparticles (MNPs), on a single filamentous fd phage, a human-safe bacteria-specific virus. The resultant phage is first decorated with MNPs and then captures the biomarker from the sera. The phage-bound biomarker is then magnetically enriched and biochemically detected. This method greatly increases the sensitivity and specificity of the biomarker detection. The average detection time for each serum sample is only about 6 h, much shorter than the clinically used gold standard method, which takes about 1 week. The detection limit of our nanobiotechnological method is approximately 1.1 pg/mL, about 2 orders of magnitude lower than that of the traditional antigen-based method, opening up a new avenue to virus-based disease diagnosis.