NEU-PROTEIN OVEREXPRESSION IN BREAST-CANCER - ASSOCIATION WITH COMEDO-TYPE DUCTAL CARCINOMA INSITU AND LIMITED PROGNOSTIC VALUE IN STAGE-II BREAST-CANCER
NEU-PROTEIN OVEREXPRESSION IN BREAST-CANCER - ASSOCIATION WITH COMEDO-TYPE DUCTAL CARCINOMA INSITU AND LIMITED PROGNOSTIC VALUE IN STAGE-II BREAST-CANCER
复制标题
DOI：
10.1056/nejm198811103191902
复制
发表时间：
1988-11-10
影响因子：
158.5
通讯作者：
NUSSE, R
中科院分区：
文献类型：
--
作者：
VANDEVIJVER, MJ;PETERSE, JL;NUSSE, R
据报道，乳腺癌中下原癌基因的扩增与有无淋巴结转移和预后不良有关。我们描述了一种免疫组织化学方法检测中性蛋白在福尔马林固定的石蜡包埋组织中的过度表达，并使用两种不同的单抗。在一组已知基因拷贝数的肿瘤中，所有neu基因扩增的肿瘤都有强烈的细胞膜染色。在189例II期乳腺癌患者中，27例(14%)有neu膜染色。Neu过度表达与较大的肿瘤大小有关(P=0.006)，而与淋巴结转移无关。在中性粒细胞过度表达的患者中(中位数随访37个月)，无病生存期没有明显缩短；这些患者的总生存期显著降低(P=0.042)，但在调整肿瘤大小后，这种减少并不明显。在45例导管原位癌中，19例(42%)有核膜染色。这19例均为大细胞粉刺生长型。在16例小细胞、乳头状或筛状生长型原位导管癌中，无一例Neu过度表达。我们得出结论，Neu过度表达可能是一种不同组织学类型乳腺癌发展的早期步骤，但我们未发现其过度表达与淋巴状态或肿瘤复发有关。《英国医学杂志》1988；319：1239-45。
Amplification of theneuproto-oncogene in breast cancer has been reported to correlate with the presence of lymph-node metastases and with a poor prognosis. We describe a method for the immunohistochemical detection of overexpression ofneuprotein on formalin-fixed paraffin-embedded tissue, with the use of two different monoclonal antibodies. In a group of tumors with a knownneu-gene copy number, intense membrane staining of tumor cells was present in all tumors withneu-gene amplification.Of 189 tumors from patients with Stage II breast cancer, 27 (14 percent) hadneu-membrane staining.Neuoverexpression was associated with larger tumor size (P = 0.006) but not with lymph-node involvement.Neu-protein expression in lymph-node metastases was the same as its expression in primary tumors. Among the patients withneuoverexpression (median follow-up, 37 months), disease-free survival was not significantly shorter; overall survival was reduced significantly in these patients (P = 0.042), but this reduction did not remain significant after adjustment for tumor size. Of 45 ductal carcinomas in situ, 19 (42 percent) hadneu-membrane staining. These 19 were all of the large-cell, comedo growth type. None of 16 ductal carcinomas in situ of small-cell, papillary, or cribriform growth type hadneuoverexpression.We conclude thatneuoverexpression may be an early step in the development of a distinct histologic type of carcinoma of the breast, but we could find no association of overexpression with lymph-node status or tumor recurrence. (N Engl J Med 1988; 319: 1239–45.)