Inhibitory effect of radiotherapy combined with weekly recombinant human endostatin on the human pulmonary adenocarcinoma A549 xenografts in nude mice
Inhibitory effect of radiotherapy combined with weekly recombinant human endostatin on the human pulmonary adenocarcinoma A549 xenografts in nude mice
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DOI：
10.1016/j.lungcan.2010.09.003
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发表时间：
2011-05-01
期刊：
影响因子：
5.3
通讯作者：
Yu, Jin-ming
中科院分区：
文献类型：
--
作者：
Jiang, Xiao-dong;Dai, Peng;Yu, Jin-ming
本研究旨在探讨放射治疗联合每周一次的重组人内皮抑素（RHES）对人肺腺癌A549裸鼠移植瘤的抑制作用。将40只A549裸鼠移植瘤模型随机分为4组，每组10只。单纯放疗组（1组）给予单纯外照射（6 MV-X线，10戈伊），瘤周皮下注射生理盐水0.2 ml，每天1次，共7 d。单用RHES组（第2组）瘤周皮下注射RHES（0.75mg/ml）0.2ml，连续7 d。联合治疗组（3组）放疗同1组，RHES同2组。对照组给予生理盐水，同1组。第3组治疗后第8天起肿瘤体积明显小于对照组（P < 0.05），第2周起肿瘤消退。治疗后第15天，第2、1、3组的肿瘤体积抑制率分别为69.65%、92.64%和116.4%，第3组的MVD数低于第1组（P < 0.05）; 2组与对照组、3组与1组VEGF表达差异无统计学意义（P > 0.05）。第3组细胞凋亡明显。放疗联合每周一次RHES治疗能明显抑制肿瘤生长，较早诱导肿瘤消退，其机制可能与改善肿瘤缺氧，抑制放疗诱导的肿瘤血管生成有关。短期应用RUES（1周）有利于临床实践。(C)2010爱思唯尔爱尔兰有限公司版权所有。
The aim of this study was to investigate the inhibitory effect of radiotherapy combined with weekly recombinant human endostatin (RHES) on the human pulmonary adenocarcinoma A549 xenografts in nude mice. The 40 A549 xenograft nude mice models were randomly divided into 4 groups (each group with 10 nude mice). Single radiotherapy group (group 1) was given a single external irradiation (6MV-X ray, 10 Gy) and peritumoral subcutaneous injection of 0.2 ml normal saline every day for 7 days. Single RUES group (group 2) was given peritumoral subcutaneous injection of 0.2 ml RHES (0.75 mg/ml) for 7 days. Combination therapy group (group 3) was given radiotherapy as the same as group 1 and RHES as the same as group 2. Control group was given normal saline as the same as group 1. The tumor volume was smaller in group 3 than in control group from the 8th day after treatment (P < 0.05) and tumor regression occurred from the second week after treatment in group 3. On the 15th day after treatment, the inhibitory rates of tumor volume were 69.65%, 92.64% and 116.4% in groups 2, 1 and 3, respectively; MVD number was lower in group 3 than in group I (P < 0.05); there was no statistical significance in VEGF expression between group 2 and control group as well as between group 3 and group 1 (P > 0.05). Apoptosis was marked in group 3. Radiotherapy combined with weekly RHES can significantly inhibit tumor growth and earlier induce tumor regression, which may be related to the improvement of tumor hypoxia and the inhibition of radiation-induced tumor angiogenesis. Short-term application (1 week) of RUES is beneficial to clinical practice. (C) 2010 Elsevier Ireland Ltd. All rights reserved.