Socially acquired nicotine self-administration with an aversive flavor cue in adolescent female rats.
Socially acquired nicotine self-administration with an aversive flavor cue in adolescent female rats.
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DOI：
10.1007/s00213-016-4249-2
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发表时间：
2016-05
影响因子：
3.4
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Wang T;Han W;Chen H
建立社会环境对啮齿动物尼古丁摄入量影响的行为模型有助于研究社会环境与吸烟之间相互作用的分子机制。我们的主要目标是通过使用厌恶的味道线索来检验尼古丁是社会获得性尼古丁静脉自我给药(IVSA)模型中主要增强剂的假设。青春期雌性大鼠被放置在装有两个舔舌计的操作式条件反射室内。可操作的舔会触发同时传递含有奎宁或糖精的味道(即味道和气味)线索以及静脉注射。静脉滴注(30μg/kg尼古丁或生理盐水)。为某些组的大鼠提供视听提示。另一只没有接受尼古丁的大鼠被放置在操作员条件反射室内，以提供中性或诱导性(即，通过饮用有味道的溶液)的社交环境。这两只大鼠被允许口面部互动的分隔物隔开。在诱导性的社会环境中，大鼠获得了稳定的尼古丁IVSA，无论是厌恶的味道线索还是欲望的味道线索，并获得了相似的输入量。中性的社会环境不支持尼古丁静脉注射，也不支持这两种线索。视听提示本身并不支持尼古丁静脉注射，但增加了尼古丁的摄入量。尼古丁增加了两只大鼠同时向中央分隔区戳鼻子的次数，这是一种社会互动的衡量标准。尽管尼古丁有负面作用，但在社会获得性尼古丁IVSA模型中，尼古丁是操纵性反应的主要增强剂。
Establishing a behavioral model for the effect of social environment on nicotine intake in rodents facilitates the investigation of molecular mechanisms critical for the interaction between social environment and cigarette smoking. Our main objective was to test the hypothesis that nicotine is the primary reinforcer in the socially acquired nicotine intravenous self-administration (IVSA) model by using an aversive flavor cue. Adolescent female rats were placed in operant conditioning chambers equipped with two lickometers. Operant licking triggered concurrent deliveries of a flavor (i.e., taste and odor) cue containing either quinine or saccharin and an i.v. infusion (30 μg/kg nicotine or saline). An audiovisual cue was provided for some groups of rats. A second rat that did not receive nicotine was placed in the operant conditioning chambers to provide either a neutral or an inducing (i.e., by consuming the flavored solution) social environment. These two rats were separated by a divider that allowed orofacial interactions. Rats acquired stable nicotine IVSA with either the aversive or the appetitive flavor cue in the inducing social environment, and obtained similar amounts of infusions. The neutral social environment did not support nicotine IVSA with either cue. The audiovisual cue per se did not support nicotine IVSA but enhanced nicotine intake. Nicotine increased the number of concurrent nose pokes by the two rats into the center divider, a measure of social interaction. Despite its aversive effects, nicotine is the primary reinforcer for the operant responses in the socially acquired nicotine IVSA model.