Fitness analyses of Arabidopsis thaliana mutants depleted of FtsH metalloproteases and characterization of three FtsH6 deletion mutants exposed to high light stress, senescence and chilling
Fitness analyses of Arabidopsis thaliana mutants depleted of FtsH metalloproteases and characterization of three FtsH6 deletion mutants exposed to high light stress, senescence and chilling
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DOI：
10.1111/j.1469-8137.2011.03684.x
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发表时间：
2011-01-01
期刊：
影响因子：
9.4
通讯作者：
Funk, Christiane
中科院分区：
文献类型：
--
作者：
Wagner, Raik;Aigner, Harald;Funk, Christiane
进行了达尔文适合度分析，通过测量植株大小、存活率、角果和种子产量，将单个FtsH突变体与在受控实验室条件下和田间生长的野生型拟南芥植物进行了比较。此外，在受控生长条件下，分析了Delta FtsH6的三种基因型在衰老和光适应期间降解光系统II捕光复合体的能力。在田间，Delta FtsH2、Delta FtsH5和Delta FtsH10突变体的多样性显著增加，生长减少；FtsH2对植物的存活尤其重要。尽管在相对寒冷的天气中生长，Delta FtsH11突变体表现出与野生型的强烈表型偏差。Delta FtsH10和Delta FtsH3突变体都表现出较轻的表型变化，但作为幼株放置在田间时与野生型植株不同。当较老的Delta FtsH3或Delta FtsH10突变体放置在室外时，没有观察到与野生型的表型差异。在控制生长条件下，在衰老和光驯化过程中，Delta FtsH6突变体的叶绿素或光系统II捕光复合体b3(Lhcb3)的数量与野生型没有差异。因此，FtsH6在体内对LHCII的降解似乎并不重要。
Darwinian fitness analyses were performed, comparing single ftsh mutants with wild-type Arabidopsis thaliana plants grown under controlled laboratory conditions and in the field, by measuring plant size, survival rate, and silique and seed production.Additionally, three genotypes of Delta FtsH6 were analysed, under controlled growth conditions, with respect to both their ability to degrade the light-harvesting complex of photosystem II during senescence and light acclimation.In the field, substantial increases in variegation and reductions in growth were observed in the Delta FtsH2, Delta FtsH5 and Delta FtsH10 mutants; FtsH2 seemed particularly important for plant survival. Despite being grown in relatively cold weather, the Delta FtsH11 mutant displayed strong phenotypic deviations from wild type. Both Delta FtsH10 and Delta FtsH3 mutants exhibited less severe phenotypic changes, but were different from wild-type plants when placed in the field as young plants. When older Delta FtsH3 or Delta FtsH10 mutants were placed outdoors, no phenotypic differences from wild type were observed. Three genotypes of Delta FtsH6 displayed no phenotypic deviations from wild-type plants.Under controlled growth conditions, during senescence and light acclimation, no differences in the amount of chlorophyll or Photosystem II light-harvesting complex b3 (Lhcb3) were detected in Delta FtsH6 mutants compared with the wild type. Therefore, FtsH6 seems to be unimportant for LHCII degradation in vivo.