Molecular Epidemiology of Extended-Spectrum β-Lactamases and Escherichia coli Isolated from Retail Foods Including Chicken Meat in Japan
Molecular Epidemiology of Extended-Spectrum β-Lactamases and Escherichia coli Isolated from Retail Foods Including Chicken Meat in Japan
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DOI：
10.1089/fpd.2013.1608
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发表时间：
2014-02-01
影响因子：
2.8
通讯作者：
Arakawa, Yoshichika
中科院分区：
文献类型：
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作者：
Kawamura, Kumiko;Goto, Kensuke;Arakawa, Yoshichika
零售肉类被产超广谱内酰胺酶（ESBL）大肠杆菌污染的报道，但在日本只有有限的数据记录。2010年1月至10月期间，从29家超市购买了153种零售食品，包括鸡肉、牛肉、猪肉和蔬菜。使用添加1 mg/L头孢噻肟的McConkey琼脂平板从每个食品样品中回收产ESBL菌。聚合酶链反应（PCR）扩增后DNA序列分析确定ESBL型别。同时测定了抗生素谱、O血清型、质粒复制子型、脉冲型和多位点序列型。从153份鸡肉样品中分离出52株产ESBL的大肠埃希菌，分离率为22.9%。ESBL类型以CTX-M-2型为主，其次为CTX-M-1型和CTX-M-8型。从进口肉类样品中检出携带bla（CTX-M-8）的菌株8株（38.1%），明显高于国产菌株1株（3.2%）（p
Contamination of retail meat with extended-spectrum -lactamase (ESBL)-producing Escherichia coli has been reported, but only limited data have been documented in Japan. One hundred fifty-three retail foods including chicken meat, beef, pork, and vegetables were purchased from 29 supermarkets between January and October in 2010. ESBL producers were recovered from each food sample using McConkey agar plate supplemented with 1mg/L of cefotaxime. ESBL type was identified by DNA sequencing analysis after polymerase chain reaction amplification. Antibiogram, O serotype, plasmid replicon type, pulsotype, and multilocus sequence type were also determined. Fifty-two epidemiologically unrelated Escherichia coli isolates producing ESBL were recovered from 35 (22.9%) of 153 samples, all of which were chicken meat. ESBL types were mainly CTX-M-2 group followed by CTX-M-1 group and CTX-M-8 group. The numbers of bacterial isolates (8 of 21, 38.1%) harboring bla(CTX-M-8) recovered from imported meat samples were significantly larger than those of domestic ones (one of 31, 3.2%) (p