Towards a saturated sorghum map using RFLP and AFLP markers
Towards a saturated sorghum map using RFLP and AFLP markers
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DOI：
10.1007/s001220051076
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发表时间：
1999-02-01
影响因子：
5.4
通讯作者：
Hamon, P
中科院分区：
文献类型：
--
作者：
Boivin, K;Deu, M;Hamon, P
利用RFLP、AFLP和形态标记构建了高粱近饱和遗传连锁图谱。首先，从两个重组近交群体的分析中获得了一个复合的，基本上基于RFLP的遗传连锁图谱。该图谱包括11个连锁群的343个位点，跨度为1352 cM。由于该图谱是用许多已作图的异源探针构建的，因此为同线性研究提供了良好的基础。对12对引物组合扩增出的168条带进行了AFLP分析，获得了一张AFLP图谱。它包括11个连锁群的137个位点，跨度849 cM。考虑到不同的数据集，我们构建了一个组合的遗传连锁图谱，包括443个位点，跨越1899 cM。主要有两个特点：（1）AFLP在基因组上的分布沿着是不均匀的;（2）在添加AFLP后，原核心图谱发生了重要的延伸。
A near-saturated sorghum genetic linkage map was produced using RFLP, AFLP and morphological markers. First a composite, essentially RFLP-based genetic linkage map was obtained from analyses of two recombinant inbred populations. This map includes 343 loci for 11 linkage groups spanning 1352 cM. Since this map was constructed with many previously mapped heterologous probes, it offers a good basis for synteny studies. Separately, an AFLP map was obtained from the analysis of 168 bands revealed from 12 primer pair combinations. It includes 137 loci for 11 linkage groups spanning 849 cM. Taking into account the different data sets, we constructed a combined genetic linkage map including 443 loci spanning 1899 cM. Two main features are to be noted: (1) the distribution of AFLPs along the genome is not uniform; (2) an important stretching of the former core map is induced after adding the AFLPs.