SPECIFIC BINDING OF THE C-PEPTIDE OF PROINSULIN TO CULTURED B-CELLS FROM A TRANSPLANTABLE RAT ISLET CELL TUMOR
SPECIFIC BINDING OF THE C-PEPTIDE OF PROINSULIN TO CULTURED B-CELLS FROM A TRANSPLANTABLE RAT ISLET CELL TUMOR
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DOI：
10.1007/bf01115006
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发表时间：
1986-02-01
影响因子：
4
通讯作者：
MARKS, V
中科院分区：
文献类型：
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作者：
FLATT, PR;SWANSTONFLATT, SK;MARKS, V
使用可移植的NEDH大鼠胰岛细胞肿瘤主要由胰岛素分泌B细胞组成，评价胰岛素原的C肽的特异性结合。培养的肿瘤B细胞表现出大于90%的生存能力，通过台盼蓝排除法评估，并保留形成肿瘤的能力，伴随着低血糖和高胰岛素血症后再植入。在用合成的大鼠C-肽I和碘化酪酰化大鼠C-肽I的结合实验中，肿瘤B-细胞表现出54 ± 0.05。6%特异性结合。随着未标记大鼠C肽I浓度的增加（0.25- 1，000 ng/ml），示踪剂的位移增加，结合特异性通过与人C肽的位移减少得到证实。特异性C肽结合的Scatchard分析显示曲线图具有向上的斜率。特异性C肽与胰岛素分泌B细胞结合的证明为胰岛素原C肽的生理作用提供了证据。
Specific binding of the C-peptide of proinsulin was evaluated using a transplanable NEDH rat islet cell tumour predominantly composed of insulin-secreting B-cells. Cultured tumour B-cells exhibited greater than 90% viability assessed by trypan blue exclusion, and retained the ability to form tumours with accompanying hypoglycaemia and hyperinsulinaemia after reimplantation. During binding experiments with synthetic rat C-peptide I and iodinated tyrosylated rat C-peptide I, tumour B-cells exhibited 54 .+-. 6% specific binding. Displacement of tracer increased with increasing concentrations of unlabelled rat C-peptide I (0.25-1,000 ng/ml), and the specificity of binding was substantiated by reduced displacement with human C-peptide. Scatchard analysis of specific C-peptide binding revealed a curvilinear plot with upward concavity. The demonstration of specific C-peptide binding to insulin-secreting B-cells provides evidence for a physiological role of proinsulin C-peptide.