Transcriptional landscape of pathogen-responsive lncRNAs in rice unveils the role of ALEX1 in jasmonate pathway and disease resistance
Transcriptional landscape of pathogen-responsive lncRNAs in rice unveils the role of ALEX1 in jasmonate pathway and disease resistance
复制标题
水稻中病原体响应lncRNA的转录格局揭示了ALEX1在茉莉酸途径和抗病性中的作用
DOI：
10.1111/pbi.13234
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发表时间：
2019-09-11
影响因子：
13.8
通讯作者：
Chen, Yue-Qin
中科院分区：
文献类型：
--
作者：
Yu, Yang;Zhou, Yan-Fei;Chen, Yue-Qin
植物防御是多层次的，对于植物在不断变化的环境中生存至关重要。长链非编码RNA（lncRNA）的发现极大地拓展了我们对多种生物学过程中转录后基因调控的理解。然而，lncRNA在植物抗病性中的表达谱和功能在很大程度上仍不明确，尤其是在单子叶植物中。在此，我们对受水稻黄单胞菌稻致病变种（Xoo）侵染的水稻叶片在不同时间进程进行链特异性RNA测序，并系统鉴定出567个响应病害的水稻lncRNA。对这些lncRNA的靶标分析表明，茉莉酸（JA）途径显著富集。为揭示lncRNA与JA相关基因之间的相互作用，我们研究了它们的共表达情况，发现39个JA相关的蛋白质编码基因与73个lncRNA存在相互作用，这突出了lncRNA在JA途径中的潜在调控作用。随后，我们鉴定出一个lncRNA——ALEX1，其表达受Xoo侵染的强烈诱导。利用增强子捕获系统构建的T-DNA插入系显示ALEX1表达量更高，且对水稻白叶枯病具有显著抗性。功能研究表明，JA信号通路被激活，JA和JA - Ile的内源含量增加。在水稻中过表达ALEX1进一步证实了JA途径的激活以及对水稻白叶枯病的抗性。我们的研究结果揭示了水稻中响应病原体的lncRNA的表达情况，并为lncRNA与JA途径在植物抗病性调控中的联系提供了新的见解。
Plant defence is multilayered and is essential for surviving in a changing environment. The discovery of long noncoding RNAs (lncRNAs) has dramatically extended our understanding of post-transcriptional gene regulation in diverse biological processes. However, the expression profile and function of lncRNAs in disease resistance are still largely unknown, especially in monocots. Here, we performed strand-specific RNA sequencing of rice leaves infected by Xanthomonas oryzae pv. Oryzae (Xoo) in different time courses and systematically identified 567 disease-responsive rice lncRNAs. Target analyses of these lncRNAs showed that jasmonate (JA) pathway was significantly enriched. To reveal the interaction between lncRNAs and JA-related genes, we studied the coexpression of them and found 39 JA-related protein-coding genes to be interplayed with 73 lncRNAs, highlighting the potential modulation of lncRNAs in JA pathway. We subsequently identified an lncRNA, ALEX1, whose expression is highly induced by Xoo infection. A T-DNA insertion line constructed using enhancer trap system showed a higher expression of ALEX1 and exerted a significant resistance to rice bacterial blight. Functional study revealed that JA signalling is activated and the endogenous content of JA and JA-Ile is increased. Overexpressing ALEX1 in rice further confirmed the activation of JA pathway and resistance to bacterial blight. Our findings reveal the expression of pathogen-responsive lncRNAs in rice and provide novel insights into the connection between lncRNAs and JA pathway in the regulation of plant disease resistance.