Nob1p, a new essential protein, associates with the 26S proteasome of growing Saccharomyces cerevisiae cells
Nob1p, a new essential protein, associates with the 26S proteasome of growing Saccharomyces cerevisiae cells
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DOI：
10.1016/s0378-1119(99)00566-1
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发表时间：
2000-02-08
期刊：
影响因子：
3.5
通讯作者：
Toh-e, A
中科院分区：
文献类型：
--
作者：
Tone, Y;Tanahashi, N;Toh-e, A
Nob1p 与 Nin1p/Rpn12（酵母 26S 蛋白酶体 19S 调节颗粒 (RP) 的亚基）相互作用，已通过双杂交筛选得到鉴定。 NOB1被发现是一个必需基因，编码459个氨基酸残基的蛋白质。在生长细胞中检测到 Nob1p，但在静止期细胞中未检测到。在过渡到稳定期期间，Nob1p 至少部分被 26S 蛋白酶体降解。 Nob1p 仅在甘油梯度离心曲线中的蛋白酶体级分中发现，并与 Rpt1（酵母蛋白酶体的 ATP 酶成分）进行免疫共沉淀。这些结果表明 Nob1p 与蛋白酶体的结合对于生长细胞中蛋白酶体的功能至关重要。 (C) 2000 Elsevier Science B.V. 保留所有权利。
Nob1p, which interacts with Nin1p/Rpn12, a subunit of the 19S regulatory particle (RP) of the yeast 26S proteasome, has been identified by two-hybrid screening. NOB1 was found to be an essential gene, encoding a protein of 459 amino acid residues. Nob1p was detected in growing cells but not in cells in the stationary phase. During the transition to the stationary phase, Nob1p was degraded, at least in part, by the 26S proteasome. Nob1p was found only in proteasomal fractions in a glycerol gradient centrifugation profile and immuno-coprecipitated with Rpt1, which is an ATPase component of the yeast proteasomes. These results suggest that association of Nob1p with the proteasomes is essential for the function of the proteasomes in growing cells. (C) 2000 Elsevier Science B.V. All rights reserved.