PATHWAYS OF INORGANIC CARBON FIXATION IN THE ENDOSYMBIONT-BEARING LUCINID CLAM LUCINOMA-AEQUIZONATA .1. PURIFICATION AND CHARACTERIZATION OF THE ENDOSYMBIOTIC BACTERIA
PATHWAYS OF INORGANIC CARBON FIXATION IN THE ENDOSYMBIONT-BEARING LUCINID CLAM LUCINOMA-AEQUIZONATA .1. PURIFICATION AND CHARACTERIZATION OF THE ENDOSYMBIOTIC BACTERIA
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DOI：
10.1002/jez.1402470102
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发表时间：
1988-07-01
影响因子：
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通讯作者：
FELBECK, H
中科院分区：
文献类型：
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作者：
DISTEL, DL;FELBECK, H
本文描述了两种从海洋双壳类红藻的细菌细胞组织中提纯硫氧化内共生菌的简单技术。从含有共生体的组织中制备组织匀浆，通过Percoll密度梯度介质的梯度离心法回收共生体。内共生菌的高密度(可能是因为细胞内存在大量硫沉积)允许这些细胞与宿主细胞和细胞成分清洁、快速和有效地分离。利用光镜和电子显微镜以及酶和生化技术对从该文蛤分离的共生菌的特性、质量和纯度进行了验证。本文所描述的技术也被成功地用于从各种海洋无脊椎动物中提纯硫氧化内共生体，包括环状双壳动物、弗罗里达纳双壳动物、华丽双壳动物和球囊尾藻，以及从vstientiferan管虫Riftia pachyptila和Tevnia Jerichonana中提纯。事实证明，这些制剂对研究这些和类似的硫氧化共生体是有用的。
Two simple techniques by which the sulfur-oxidizing endosymbiotic bacteria may be purified from the bacteriocyte tissues of the marine bivalve Lucinoma aequizonata are described. Tissue homogenates are prepared from symbiont-containing tissues, and symbionts are recovered by centrifugation through gradients of Percoll density gradient medium. The high density of the endosymbionts (probably because of the presence of numerous sulfur deposits within the cells) allows clean, rapid, and efficient separation of these cells from host cells and cell constituents. Light and electron microscopy and enzymatic and biochemical techniques are used to demonstrate the identity, quality, and purity of the symbiont isolates from this lucinid clam. The techniques described here for L. aequizonata have also been used successfully to purify sulfur-oxidizing endosymbionts from a variety of marine invertebrates, including the bivalves L. annulata, Lucina floridana, Calyptogena magnifica, and Codakia orbicularis and from the vestimentiferan tubeworms Riftia pachyptila and Tevnia jerichonana. Such preparations should prove useful for investigating these and similar sulfur-oxidizing symbioses.