17β-estradiol differently affects osteogenic differentiation of mesenchymal stem/stromal cells from adipose tissue and bone marrow
17β-estradiol differently affects osteogenic differentiation of mesenchymal stem/stromal cells from adipose tissue and bone marrow
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DOI：
10.1016/j.diff.2016.04.001
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发表时间：
2016-12-01
期刊：
影响因子：
2.9
通讯作者：
Brini, Anna Teresa
中科院分区：
文献类型：
--
作者：
Niada, Stefania;Giannasi, Chiara;Brini, Anna Teresa
脂肪源性和骨髓干细胞/基质细胞（ASCs和BMSCs）在再生医学中的应用经常被比较，并且对维持其分化和功效的几个因素进行了研究。据报道，β -雌二醇（E2）可影响祖细胞的某些功能。本研究研究了10和100 nM E2对ASC和BMSC活力、增殖和向成骨和成脂谱系分化的影响。E2不调节ASC和BMSC的活力和生长速度，而激素对间充质干细胞/基质细胞（MSCs）产生促脂肪作用。特别是，7天前处理和100 nM E2之间的协同作用导致了最明显的结果，ASCs和BMSCs中脂泡形成分别增加了+44%和+82%。尽管E2没有改变骨诱导间充质干细胞的胶原沉积，但我们观察到ASC和BMSC碱性磷酸酶（ALP）活性的不同调节。事实上，17 - β -雌二醇在骨髓间充质干细胞中始终增强了这一成骨标志物，100 nM E2预处理7天使其增加了约70%。相反，E2削弱ASC的成骨潜能，使其ALP活性降低约20%，其中对绝经前妇女分离的ASC的影响最为明显（-30%）。最后，我们确定了在两种间充质干细胞中表达的约37 kDa的雌激素受体α (ER α)变体。有趣的是，脂肪生成刺激显著降低其表达，而成骨刺激仅在骨髓间充质干细胞中轻度增加其表达。综上所述，E2对两种间充质干细胞的成脂过程均有积极影响，但仅对骨髓间充质干细胞具有成骨诱导作用，两种间充质祖细胞均表达了一种新的37 kDa er - α变体，其表达在分化过程中受到调节。(C) 2016年国际分化学会。Elsevier B.V.版权所有。
Adipose-derived and bone marrow stem/stromal cells (ASCs and BMSCs) have been often compared for their application in regenerative medicine, and several factors sustaining their differentiation and efficacy have been investigated. 17 beta-estradiol (E2) has been reported to influence some functions of progenitor cells. Here we studied the effects of 10 and 100 nM E2 on ASC and BMSC vitality, proliferation and differentiation towards osteogenic and adipogenic lineages. E2 did not modulate ASC and BMSC vitality and growth rate, while the hormone produced a pro-adipogenic effect on both mesenchymal stem/ stromal cells (MSCs). In particular, the synergy between 7-day pre-treatment and 100 nM E2 led to the most evident result, increasing lipid vacuoles formation in ASCs and BMSCs of +44% and +82%, respectively. Despite the fact that E2 did not alter collagen deposition of osteo-induced MSCs, we observed a different modulation of ASC and BMSC alkaline phosphatase (ALP) activity. Indeed, this osteogenic marker was always enhanced by 17 beta-estradiol in BMSCs, and 7-day pre-treatment with 100 nM E2 increased it of about 70%. In contrast, E2 weakened ASC osteogenic potential, reducing their ALP activity of about 20%, with the most evident effect on ASCs isolated from pre-menopausal women (-30%).Finally, we identified an estrogen receptor alpha (ER alpha) variant of about 37 kDa expressed in both MSCs. Interestingly, adipogenic stimuli drastically reduced its expression, while osteogenic ones mildly increased this isoform in BMSCs only.In conclusion, E2 positively affected the adipogenic process of both MSCs while it favored osteogenic induction in BMSCs only, and both mesenchymal progenitors expressed a novel 37 kDa ER-alpha variant whose expression was modulated during differentiation. (C) 2016 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.