[Antigenic types of Rickettsia tsutsugamushi isolated from patients with tsutsugamushi fever and their distribution in Miyazaki Prefecture].
[Antigenic types of Rickettsia tsutsugamushi isolated from patients with tsutsugamushi fever and their distribution in Miyazaki Prefecture].
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恙虫病患者中分离的恙虫病立克次体的抗原类型及其在宫崎县的分布[J].
DOI：
10.11150/kansenshogakuzasshi1970.63.109
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Y. Minamishima
中科院分区：
文献类型：
--
作者：
S. Yamamoto;N. Kawabata;K. Ooura;M. Murata;Y. Minamishima
对恙虫热患者分离的恙虫立克次体（Rt）进行了抗原性检测。这是通过间接免疫荧光（IIF），用1981年分离的针对三种标准菌株（Karp， Kato和Gilliam）和两种当地菌株（Kawasaki和Kuroki）的豚鼠抗血清和针对三种标准菌株的小鼠单克隆抗体来完成的。与此同时，在1985年至1988年期间登记的442名患者中，有317名患者的血清抗体用IIF滴定了这5种Rt菌株。1)本地分离株川崎（Kawasaki）和黑木（Kuroki）菌株分别与同源抗血清反应最有效，对异源抗血清的IIF滴度降低了4倍。2)川崎株对抗karp和抗kato单克隆抗体均无反应，黑木株对抗gilliam单克隆抗体无反应。3) 1985年分离的27株株中，17株对抗血清和单克隆抗体的反应与川崎株相似，其余10株的反应与黑木株相似。4) 317例患者中233例（74%）的血清抗体效价最高，317例患者中69例（22%）的血清抗体效价最高。由此可见，川崎株和黑木株在抗原性上与标准株存在差异，川崎株和黑木株之间也存在差异。提示宫崎县存在川崎型和黑崎型两种抗原型Rt，川崎型Rt略占优势，近期恙虫病热可能是由其中一种或另一种Rt引起的。
Rickettsia tsutsugamushi (Rt) isolated from patients with tsutsugamushi fever were examined for their antigenicity. This was done by indirect immunofluorescence (IIF) with guinea pig antisera against three standard strains (Karp, Kato and Gilliam) and two local strains (Kawasaki and Kuroki) isolated in 1981, and with mouse monoclonal antibodies against the three standard strains. In the meantime, antibodies in sera from 317 out of 442 patients registered during 1985 to 1988 were titrated by IIF with those five Rt strains. 1) Local isolates, Kawasaki and Kuroki strains, reacted most effectively with the homologous antiserum, respectively, showing four fold lower IIF titers against the heterologous antisera. 2) Kawasaki strain reacted with none of the monoclonal antibodies, whereas Kuroki strain showed a slight reaction with anti-Karp and anti-Kato, but not anti-Gilliam, monoclonal antibodies. 3) Seventeen out of 27 strains isolated in 1985 resembled the Kawasaki strain in their reaction patterns with the antisera and monoclonal antibodies, and the other 10 strains showed reactivity similar to the Kuroki strain. 4) Sera of 233 (74%) out of 317 patients showed the highest antibody titers against the Kawasaki strain and 69 (22%) of 317 against the Kuroki strain. It is thus evident that Kawasaki and Kuroki strains are antigenically different from the standard strains, and Kawasaki and Kuroki strains also differ from each other. It is suggested that two antigenic types (Kawasaki and Kuroki) of Rt were distributed in Miyazaki Prefecture, Rt of the Kawasaki type slightly dominates Rt of the Kuroki type, and recent tsutsugamushi fever has been caused by either one or the other type of Rt.