The draft genome sequence of the Japanese honey bee, Apis cerana japonica (Hymenoptera: Apidae)
The draft genome sequence of the Japanese honey bee, Apis cerana japonica (Hymenoptera: Apidae)
复制标题
DOI：
10.14411/eje.2018.064
复制
发表时间：
2018-11
影响因子：
1.3
通讯作者：
K. Yokoi;H. Uchiyama;T. Wakamiya;M. Yoshiyama;J. Takahashi;T. Nomura;Tsutomu Furukawa;S. Yajima;K. Kimura
中科院分区：
文献类型：
--
作者：
K. Yokoi;H. Uchiyama;T. Wakamiya;M. Yoshiyama;J. Takahashi;T. Nomura;Tsutomu Furukawa;S. Yajima;K. Kimura
蜜蜂不仅对蜂蜜生产很重要，而且还是野生和栽培植物的授粉者。东方蜜蜂(Apis Cerana)对几种病原菌的抗性比西方蜜蜂(Apis Melvena)强，并测定了东方蜜蜂北方(韩国)和南方(中国)两个非典型亚种的基因组。中华蜜蜂是中华蜜蜂的另一个亚种，表现出许多特殊的特征(如温和、产蜜少和经常潜逃)，研究这些特征的分子生物学和遗传学方面具有重要意义。为了加快中华绒螯蟹的遗传研究，我们对该亚种的基因组进行了测序。在基本基因组状态(如N50为180KBP，全长为211MBP，最大重叠群长度为1.31MBP)和BUSCO结果方面，本文所提供的中华鳖基因组草稿序列是高质量的。用Augustus软件预测中华绒螯蟹的基因集，用Blastp和InterProScan对该基因集进行注释，并在每个基因上添加GO项。该基因的数量高于此前测序的意大利蜜蜂和中华蜜蜂的两个品系。在中华蜜蜂基因组中发现了少量的转座元件和重复区，它们也存在于意大利蜜蜂和中华东方蜜蜂的北方和南方品系的基因组中。中华蜜蜂对几种严重危害意大利蜜蜂的病原菌具有抗性。我们搜索了41个与IMD和Toll通路相关的同源基因，它们在对入侵病原体的免疫反应中起着关键作用。在中华按蚊北方株基因组中未发现一些同源基因。这表明Toll和IMD通路的功能与意大利蜜蜂和黑腹果蝇的功能相同。使用本文提供的中华蜜蜂基因组草稿序列和其他蜜蜂(亚种)的基因组草稿序列可以帮助加快对蜜蜂基因组的比较研究。
Honey bees are not only important for honey production but also as pollinators of wild and cultivated plants. The Eastern honeybee (Apis cerana) is more resistant to several pathogens than the Western honeybee (Apis mellifera), and the genomes of two strains of the nominotypical subspecies, A. cerana cerana, northern (Korea) and southern (China) strains, have been sequenced. Apis cerana japonica, another subspecies of A. cerana, shows many specific features (e.g. mildness, low honey production and frequently absconds) and it is important to study the molecular biological and genetic aspects of these features. To accelerate the genetic research on A. cerana japonica, we sequenced the genome of this subspecies. The draft genome sequence of A. cerana japonica presented here is of high quality in terms of basic genome status (e.g. N50 is 180 kbp, total length is 211 Mbp, and largest contig length is 1.31 Mbp) and BUSCO results. The gene set of A. cerana japonica was predicted using AUGUSTUS software and the set of genes was annotated using Blastp and InterProScan, and GO terms were added to each gene. The number of genes is higher than in A. mellifera and in the two strains of A. cerana cerana sequenced previously. A small number of transposable elements and repetitive regions were found in A. cerana japonica, which are also in the genomes of A. mellifera and the northern and southern strains of A. cerana cerana. Apis cerana is resistant to several pathogens that seriously damage A. mellifera. We searched for 41 orthologs related to the IMD and Toll pathways, which have key roles in the immune reaction to invading pathogens. Some orthologs were not identified in the genome of the northern strain of A. cerana cerana. This indicates that the Toll and IMD pathways function in the same way as in A. mellifera and Drosophila melanogaster. Use of the draft genome sequence of A. cerana japonica provided herein and those of the other Apis (sub)species may help to accelerate comparative research on the genome of honey bees.