Confirmation of Borrelia burgdorferi spirochetes by polymerase chain reaction in placentas of women with reactive serology for Lyme antibodies.
Confirmation of Borrelia burgdorferi spirochetes by polymerase chain reaction in placentas of women with reactive serology for Lyme antibodies.
复制标题
通过莱姆病抗体反应性血清学检测的妇女胎盘中的聚合酶链反应确认伯氏疏螺旋体螺旋体。
DOI：
10.1159/000292277
复制
发表时间：
1996
影响因子：
2.1
通讯作者：
Schwartz,I
中科院分区：
文献类型：
--
作者：
Figueroa,R;Bracero,LA;Aguero-Rosenfeld,M;Beneck,D;Coleman,J;Schwartz,I
本研究的目的是使用银染法确定莱姆病血清学反应的无症状妇女胎盘中是否存在伯氏疏螺旋体螺旋体，并通过聚合酶链反应（PCR）确认螺旋体的身份。用银染法对60例妊娠期莱姆病抗体ELISA阳性或可疑血清学无症状妇女的胎盘进行螺旋体检查。ELISA血清学结果通过Western印迹分析证实。B.对鉴定为具有螺旋体的胎盘和一组螺旋体阴性的胎盘进行Burgdorferi。银染法在3/60个胎盘中鉴定出螺旋体（5%）。PCR证实B.其中两个胎盘中存在伯氏脱氧核糖核酸序列。3名妇女的莱姆病ELISA结果可疑，梅毒血清学阴性。免疫印迹分析2例阴性，1例不确定。通过银染色和PCR，6个对照为螺旋体阴性。在所有病例中均观察到正常的围产期结局。在莱姆病血清学反应孕妇胎盘组织中发现的螺旋体经PCR证实为beB。burgdorferi。胎盘螺旋体的存在与莱姆病血清学结果或妊娠结局无关。
The purpose of our study was to determine whetherBorrelia burgdorferispirochetes were present in placentas of asymptomatic women with reactive Lyme serology using a silver stain, and to confirm the identity of the spirochetes by polymerase chain reaction (PCR). Sixty placentas of asymptomatic women with ELISA-positive or -equivocal serology for Lyme antibodies during pregnancy were examined for spirochetes using a silver stain. The results of the ELISA serology were confirmed by Western blot analysis. PCR amplification forB. burgdorferiwas performed on placentas identified to have spirochetes and on a group of placentas negative for spirochetes. Spirochetes were identified by silver staining in 3 (5%) of the 60 placentas. PCR confirmedB. burgdorferinucleotide sequences in 2 of the placentas. The 3 women had equivocal Lyme ELISA and negative syphilis serology. The results of the Western blot analysis were negative in 2 cases and indeterminate in 1 case. Six controls were negative for spirochetes by silver staining and PCR. A normal perinatal outcome was observed in all cases. Spirochetes identified in placental tissue of pregnancies with reactive Lyme serology were confirmed by PCR to beB. burgdorferi. There was no relationship between the presence of placental spirochetes and the results of Lyme serology or the pregnancy outcome.