Impact of oestradiol and progesterone on antioxidant activity in normal human breast epithelial cells in culture
Impact of oestradiol and progesterone on antioxidant activity in normal human breast epithelial cells in culture
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DOI：
10.3109/10715769809069276
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发表时间：
1998-01-01
影响因子：
3.3
通讯作者：
Öllinger, K
中科院分区：
文献类型：
--
作者：
Dabrosin, C;Hammar, M;Öllinger, K
长期使用雌激素和孕激素会增加患乳腺癌的风险，乳腺癌的发生部分是由于DNA碱基的氧化损伤。因此，我们研究了17 β-雌二醇和孕酮对培养的正常人乳腺上皮细胞的抗氧化状态和对氧化应激的脆弱性的影响。暴露于过氧化氢后，细胞生长与雌二醇单独或与雌二醇和孕酮显着降低的活力相比，细胞生长在培养基中没有添加激素。然而，对照组和激素处理的培养物之间的过氧化氢降解率没有差异。当加入去铁胺时，活性增加，过氧化氢降解速率降低。在雌二醇和孕酮存在下生长的细胞中，几种抗氧化剂的水平发生了变化：谷胱甘肽还原酶和过氧化氢酶的浓度显着下降，而谷胱甘肽过氧化物酶和还原型谷胱甘肽的水平没有变化。在酶活性和细胞脆弱性的改变更明显的雌二醇和孕酮的组合处理的文化，我们得出结论，在培养的正常人乳腺上皮细胞的氧化还原平衡被改变了治疗与雌二醇和孕酮，这种变化导致随后暴露于过氧化氢的细胞死亡增加。这种效应可能对乳腺性激素依赖性疾病有影响。
The risk of developing breast cancer increases after long term use of oestrogen and progestagen, and carcinogenesis in the breast is partly due to oxidative damage to DNA bases. Therefore, we studied the effects of 17 beta-oestradiol and progesterone on the antioxidative status and the vulnerability to oxidative stress exhibited by normal human breast epithelial cells in culture. After exposure to hydrogen peroxide, cells grown with oestradiol alone or with both oestradiol and progesterone showed significantly decreased viability compared to cells grown in medium without added hormones. There was, however, no difference in hydrogen peroxide degradation rate between controls and hormone treated cultures. When desferrioxamine was added, the viability increased and the hydrogen peroxide degradation rate decreased. The levels of several antioxidants were altered in cells grown in the presence of oestradiol and progesterone: the concentrations of glutathione reductase and catalase decreased significantly while the levels of glutathione peroxidase and reduced glutathione did not change. The alterations in enzyme activity and cell vulnerability were more pronounced in cultures treated with a combination of oestradiol and progesterone.We conclude that the redox balance in the cultured normal human breast epithelial cells was altered by treatment with oestradiol and progesterone, and that this change led to the increased death of cells subsequently exposed to hydrogen peroxide. This effect may have implications for sex hormone dependent diseases of the breast.