Intracellular mechanisms underlying prostaglandin F2alpha-stimulated phasic myometrial contractions.
Intracellular mechanisms underlying prostaglandin F2alpha-stimulated phasic myometrial contractions.
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前列腺素 F2α 刺激阶段性子宫肌层收缩的细胞内机制。
DOI：
10.1152/ajpendo.1997.273.4.e665
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Basa,A
中科院分区：
文献类型：
--
作者：
Phillippe,M;Saunders,T;Basa,A
这些研究旨在检验以下假设：前列腺素F2α（PGF2α）刺激的阶段性子宫肌层收缩的特征在于磷脂酰肌醇信号通路的激活，导致细胞溶质钙振荡的产生。对于本报告中描述的实验，在组织加载Fura 2后，使用大鼠子宫肌层组织进行细胞溶质钙成像研究并进行计算机数字化体外等长收缩研究。与上述假设一致，细胞溶质钙成像研究表明PGF 2 α刺激的细胞溶质钙振荡与相位收缩同时发生。体外等长收缩研究证实，先前报道的磷脂酰肌醇信号通路和胞浆钙振荡机制抑制剂可显著抑制PGF 2 α刺激的阶段性子宫肌收缩。总之，这些研究为以下假设提供了实质性支持：PGF 2 α刺激的阶段性子宫肌层收缩是由细胞内信号传导机制产生的，涉及磷脂酰肌醇信号传导途径的激活和胞浆钙振荡样现象的产生。
These studies sought to test the hypothesis that prostaglandin F2α(PGF2α)-stimulated phasic myometrial contractions are characterized by the activation of the phosphatidylinositol-signaling pathway resulting in the generation of cytosolic calcium oscillations. For the experiments described in this report rat myometrial tissue was used, after the tissue was loaded with fura 2, to perform cytosolic calcium imaging studies and to perform computer-digitalized in vitro isometric contraction studies. Consistent with the above hypothesis, the cytosolic calcium-imaging studies demonstrated PGF2α-stimulated cytosolic calcium oscillations occurring simultaneously with phasic contractions. The in vitro isometric contraction studies confirmed that previously reported inhibitors of the phosphatidylinositol-signaling pathway and cytosolic calcium oscillation mechanisms resulted in significant inhibition of PGF2α-stimulated phasic myometrial contractions. In summary, these studies have provided substantial support for the hypothesis that PGF2α-stimulated phasic myometrial contractions are generated by intracellular signaling mechanisms involving activation of the phosphatidylinositol-signaling pathway and the production of cytosolic calcium oscillation-like phenomena.