The dynamic stator stalk of rotary ATPases.
The dynamic stator stalk of rotary ATPases.
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DOI：
10.1038/ncomms1693
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发表时间：
2012-02-21
影响因子：
16.6
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中科院分区：
文献类型：
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旋转ATP酶将ATP水解/合成与跨生物膜的质子移位结合在一起，因此是生物能量转换机制的核心组成部分。它们的外围茎是抵消ATP合成或水解过程中中心茎旋转产生的扭矩的重要组成部分。在这里，我们展示了一个2.25-Å分辨率的来自嗜热热菌a型atp酶/合成酶的外周茎的晶体结构。我们确定了结构内部固有的弯曲和扭曲运动，以适应和补充atp酶头群在催化循环过程中的径向摆动，同时仍然保持抵消中心柄旋转所需的方位刚度。外周茎的构象自由是由其不寻常的右旋盘绕结构决定的，原则上在所有旋转atp酶中都是保守的。在完整酶的情况下，外周茎的动力学为旋转atp酶的远端部分之间的合作提供了潜在的机制。在ATP合成或水解过程中，旋转ATP酶的外围杆抵消由中心杆旋转产生的扭矩。Stewart等人报道了a型atp酶/合成酶外周柄的晶体结构，并确定了允许头群径向摆动的弯曲和扭转运动。
Rotary ATPases couple ATP hydrolysis/synthesis with proton translocation across biological membranes and so are central components of the biological energy conversion machinery. Their peripheral stalks are essential components that counteract torque generated by rotation of the central stalk during ATP synthesis or hydrolysis. Here we present a 2.25-Å resolution crystal structure of the peripheral stalk from Thermus thermophilus A-type ATPase/synthase. We identify bending and twisting motions inherent within the structure that accommodate and complement a radial wobbling of the ATPase headgroup as it progresses through its catalytic cycles, while still retaining azimuthal stiffness necessary to counteract rotation of the central stalk. The conformational freedom of the peripheral stalk is dictated by its unusual right-handed coiled-coil architecture, which is in principle conserved across all rotary ATPases. In context of the intact enzyme, the dynamics of the peripheral stalks provides a potential mechanism for cooperativity between distant parts of rotary ATPases. The peripheral stalks of rotary ATPases counteract torque generated by rotation of the central stalk during ATP synthesis or hydrolysis. Stewart et al. report the crystal structure of an A-type ATPase/synthase peripheral stalk and identify bending and twisting motions that permit the radial wobbling of the headgroup.
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DOI：
10.1107/s0907444905036693
发表时间：
2006-01-01
影响因子：
2.2
作者：
Evans, P
通讯作者：
Evans, P
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
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通讯作者：
Cowtan, K
影响因子：
3.5
作者：
Cross, RL;Müller, V
通讯作者：
Müller, V
影响因子：
2.9
作者：
Krebs, WG;Alexandrov, V;Gerstein, M
通讯作者：
Gerstein, M
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH