A filter paper sampling method for the uric acid:creatinine ratio in urine. Normal values in the the newborn.
A filter paper sampling method for the uric acid:creatinine ratio in urine. Normal values in the the newborn.
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尿液中尿酸：肌酐比值的滤纸采样方法
DOI：
10.1542/peds.49.1.80
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发表时间：
1972
期刊：
影响因子：
8
通讯作者：
C R Scriver
中科院分区：
文献类型：
--
作者：
Roderick Mclnnes;Peter Lamm;C. Clow;C R Scriver
本文介绍了一种测定滤纸上收集的尿液中尿酸与肌酐比值（UA/C比值）的简便方法。尿酸和肌酸酐可以在碱性缓冲液中从Schleicher和Schuell 903滤纸上定量洗脱;通过自动化方法同时分析尿液中的两种化合物。
测定了820例新生儿出生后的平均UA/C比值。第三天后，平均值为1.49 ± 0.60（平均值± 1 SD），从第二天的2.25 ± 0.85的高值下降。在这个年龄没有显著的性别差异或昼夜变化，胎龄和出生体重都没有明显影响比值。
该技术可用于筛查人群，特别是出生后第3天的新生儿嘌呤代谢紊乱。异常比率（即，高于3.29或高于平均值> 3SD）将指示这种病症。至少有0.5%的样本可出现短暂的高UA/C比值，通常是由于获得性围产期并发症。
A simple method is described for determining the uric acid: creatinine ratio (UA/C ratio) in urine collected on filter paper. Uric acid and creatinine can be eluted quantitatively from Schleicher and Schuell 903 filter paper in alkaline buffer; the eluate is analyzed for the two compounds simultaneously by an automated method.
The mean UA/C ratio was defined in 820 newborn infants in the postnatal period of life. After the third day, the average is 1.49 ± 0.60 (mean ± 1SD), falling from a high value of 2.25 ± 0.85 on the second day. There is no significant sex difference or diurnal variation at this age and neither gestational age nor birth weight apparently affects the ratio.
The technique can be used to screen population, particularly the neonate after the 3rd day of life, for disorders of purine metabolism. An abnormal ratio (i.e., above 3.29 or > 3SD above the mean) would indicate such a disorder. Transient high UA/C ratios can be anticipated in at least 0.5% of samples often because of acquired perinatal complications.