Inhibition of Enzyme Secretion in Plant Pathogens by Mepanipyrim, a Novel Fungicide
Inhibition of Enzyme Secretion in Plant Pathogens by Mepanipyrim, a Novel Fungicide
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新型杀菌剂嘧菌胺抑制植物病原菌酶分泌
DOI：
10.1006/pest.1994.1023
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发表时间：
1994
影响因子：
4.7
通讯作者：
I. Yamaguchi
中科院分区：
文献类型：
--
作者：
I. Miura;T. Kamakura;S. Maeno;S. Hayashi;I. Yamaguchi
摘要 Mepanipyrim，N-(4-methyl-6-prop-1-ynylpyrimidin-2-yl) aniline，强烈影响灰霉菌（灰霉病的致病真菌）的感染过程。嘧菌胺不抑制灰霉病宿主细胞壁降解酶（角质酶、果胶酶、纤维素酶）的活性，这些酶在感染过程中发挥重要作用。然而，它阻止了这些酶的分泌，导致它们在细胞内积累。宿主细胞壁降解酶的分泌抑制被认为会导致感染活性的抑制，并且这被认为是嘧菌胺疾病控制的重要机制。嘧菌胺还可阻止灰霉病菌脂肪酶、蛋白酶和转化酶的分泌。结果表明嘧菌胺影响分泌蛋白的细胞内转运过程。
Abstract Mepanipyrim, N -(4-methyl-6-prop-1-ynylpyrimidin-2-yl) aniline, strongly affected the infection process of Botrytis cinerea , a causative fungus of gray mold diseases. Mepanipyrim did not inhibit the activity of host-cell wall degrading enzymes (cutinase, pectinase, cellulase) of B. cinerea , which play an important role in the infection process. However, it prevented the secretion of these enzymes, leading to their intracellular accumulation. Secretory repression of host-cell wall degrading enzymes is assumed to cause depression of infectious activity and it is suggested to be an important mechanism in mepanipyrim disease control. Mepanipyrim also prevented the secretion of lipase, protease, and invertase of B. cinerea . The results suggest that mepanipyrim affects the intracellular transport process of secretory proteins.