Transcriptomic and immunohistochemical approaches identify HLA-G as a predictive biomarker of gestational choriocarcinoma resistance to monochemotherapy
Transcriptomic and immunohistochemical approaches identify HLA-G as a predictive biomarker of gestational choriocarcinoma resistance to monochemotherapy
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DOI：
10.1016/j.ygyno.2020.05.042
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发表时间：
2020-09-01
影响因子：
4.7
通讯作者：
Mallet, Francois
中科院分区：
文献类型：
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作者：
Bolze, Pierre-Adrien;Lopez, Jonathan;Mallet, Francois
目标。通过对组织样本的转录方法，我们试图确定磨牙后恶性转化的预测性生物标志物，以及毛毛癌对单一（甲氨蝶呤或放线菌素D）或多种化疗方案（EMA（依托泊苷，甲氨蝶呤，放线菌素D）-CO（环磷酰胺，长春新碱）和EMA- ep（依托泊苷，顺铂））的化学敏感性。我们研究了一个760个基因面板（PanCancer Pathway）在完全包膜样痣和妊娠绒毛膜癌组织样本中与肿瘤发生和免疫耐受相关的表达。我们没有发现绒毛膜癌臼齿后恶性转化痣（n = 14）和缓解痣（n = 20）之间有任何差异表达的基因。在单耐药绒毛膜癌（n = 34）中，4个基因（HLA-G、COL27A1、IL1R2和GLI3）表达显著降低，1个基因（THEM4）表达升高[FDR（假发现率）调整p值]
Objective. Using a transcriptional approach on tissue samples, we sought to identify predictive biomarkers of post molar malignant transformation, and of choriocarcinoma chemosensitivity to mono(methotrexate or actinomycin D) or polychemotherapy [EMA(Etoposide, Methotrexate, Actinomycin D)-CO(Cyclophosphamide, Vincristine) and EMA-EP(Etoposide, Cisplatine)] regimens.Methods. We studied the expression of a 760-gene panel (PanCancer Pathway) related to oncogenesis and immune tolerance in tissue samples of complete hydatidiform moles and gestational choriocarcinoma.Results. We did not identify any differentially expressed gene between moles with post molar malignant transformation in choriocarcinoma (n = 14) and moles with remission (n = 20). In monochemoresistant choriocarcinoma (n = 34), four genes (HLA-G, COL27A1, IL1R2 and GLI3) had a significantly reduced expression and one (THEM4) had an increased expression [FDR (false discovery rate) adjusted p-value