A homeodomain-zinc finger protein, ZFHX4, is expressed in neuronal differentiation manner and suppressed in muscle differentiation manner
A homeodomain-zinc finger protein, ZFHX4, is expressed in neuronal differentiation manner and suppressed in muscle differentiation manner
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DOI：
10.1248/bpb.29.1830
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发表时间：
2006-09-01
影响因子：
2
通讯作者：
Tsuchiya, Kayoko
中科院分区：
文献类型：
--
作者：
Hemmi, Kazunori;Ma, Dongping;Tsuchiya, Kayoko
人类ZFHX4最近被证明是先天性双侧孤立性上睑下垂的候选基因。本文报道了人ZFHX4基因cDNA的分子克隆和该基因的基因组组织。人类ZFHX4全长约180kb，包含12个外显子，编码一个含有3599个氨基酸的蛋白，携带4个同源结构域和22个锌指。第11个外显子长度为3.2 kb，与22个锌指中的4个一起编码所有4个同源结构域。ZFHX4与小鼠ZFHX4同源90%，与人类ATBF1A同源52%，与果蝇ZFH-2同源24%。以BAC克隆R-P11-48D4为探针，采用荧光原位杂交技术将ZFDX4定位于人类染色体8q13.3-q21.11。RT-PCR分析显示，ZFHX4转录本在成人大脑、肝脏和肌肉中均有表达。再加上发现Zfhx4在分化P19胚胎癌细胞和C2C12成肌细胞中有短暂表达，提示Zfhx4 /Zfhx4参与神经和肌肉的分化。
Human ZFHX4 has recently been shown to be a candidate gene for congenital bilateral isolated ptosis. Here, we report molecular cloning of the human ZFHX4 cDNA and genomic organization of this gene. Human ZFHX4 is about 180 kb long, containing 12 exons that encodes a 3599-amino acid protein carrying four homeodomains and 22 zinc fingers. The 11th exon is 3.2 kb in length and encodes all the four homeodomains together with four of the 22 zinc fingers. ZFHX4 is 90% homologous to mouse Zfhx4, 52% to human ATBF1A and 24% to Drosophila ZFH-2. ZFDX4 was mapped to human chromosome 8q13.3-q21.11 by fluorescence in situ hybridization using BAC clone R-P11-48D4 as a probe. RT-PCR analysis showed that ZFHX4 transcripts were expressed in adult human brain, liver and muscle. This, together with the finding that Zfhx4 was expressed transiently in differentiating P19 embryonal carcinoma cells and C2C12 myoblasts, suggests that ZFHX4/Zfhx4 is involved in neural and muscle differentiation.